Cloning and characterization of a surface antigen of Eimeria tenella merozoites and expression using a recombinant vaccinia virus
| Autor: | Gilbert Weber, Binger Mary-Helen, Eugene G Schildknecht, Luis Pasamontes, Markus Hümbelin, Denis Hug |
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| Rok vydání: | 1993 |
| Předmět: |
Molecular Sequence Data
Restriction Mapping Antigens Protozoan Vaccinia virus Biology Transfection Virus Eimeria law.invention Cell Line Open Reading Frames law Escherichia coli Animals Genomic library Poxviridae Orthopoxvirus Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Gene Library Recombination Genetic Base Sequence cDNA library DNA Protozoan biology.organism_classification Virology Molecular biology Recombinant Proteins Molecular Weight Open reading frame Protein Biosynthesis Recombinant DNA Parasitology Eimeria tenella RNA Protozoan Plasmids |
| Zdroj: | Molecular and biochemical parasitology. 61(2) |
| ISSN: | 0166-6851 |
| Popis: | A rabbit serum raised against Eimeria tenella merozoites was used to screen a lambda gt11 cDNA library made from merozoite mRNA of E. tenella. The insert of the phage clone lambda Mz 5-7 revealed an open reading frame consisting of 945 nucleotides, encoding a 33-kDa protein. This size is consistent with the size of a protein translated in vitro from merozoite mRNA and immunoprecipitated with monospecific anti-Mzp 5-7 antibodies. A smaller protein of 24 kDa, located on the surface of the parasite, also reacted with the monospecific antiserum and is the potential processed form of the Mzp 5-7. Furthermore, a recombinant vaccinia virus expressing the Mzp 5-7 antigen was constructed and used to immunize chickens. |
| Databáze: | OpenAIRE |
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