Induction of Type 2 Iodothyronine Deiodinase After Status Epilepticus Modifies Hippocampal Gene Expression in Male Mice
| Autor: | Roberta Monterazzo Cysneiros, Eduardo Fernandes Bondan, Antonio C. Bianco, Barbara M L C Bocco, Gustavo W. Fernandes, Tatiana L. Fonseca, C.V. Cardoso, Elizabeth A. McAninch, Miriam O. Ribeiro, Renata J Nassif, Bruna Pascarelli Pedrico do Nascimento |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Programmed cell death Deiodinase Hippocampus DIO2 Cellular homeostasis Gene Expression Prefrontal Cortex Apoptosis Status epilepticus Hippocampal formation Muscarinic Agonists Iodide Peroxidase 03 medical and health sciences Mice 0302 clinical medicine Endocrinology Status Epilepticus Internal medicine medicine Animals Research Articles Cell Nucleus Inflammation Mice Knockout Neurons biology Cell Death Pilocarpine Amygdala Oxidative Stress 030104 developmental biology Iodothyronine deiodinase Astrocytes biology.protein Triiodothyronine medicine.symptom 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Endocrinology. 159(8) |
| ISSN: | 1945-7170 |
| Popis: | Status epilepticus (SE) is an abnormally prolonged seizure that results from either a failure of mechanisms that terminate seizures or from initiating mechanisms that inherently lead to prolonged seizures. Here we report that mice experiencing a 3 hours of SE caused by pilocarpine exhibit a rapid increase in expression of type 2 iodothyronine deiodinase gene (Dio2) and a decrease in the expression of type 3 iodothyronine deiodinase gene in hippocampus, amygdala and prefrontal cortex. Type 3 iodothyronine deiodinase in hippocampal sections was seen concentrated in the neuronal nuclei, typical of ischemic injury of the brain. An unbiased analysis of the hippocampal transcriptome of mice undergoing 3 hours of SE revealed a number of genes, including those involved with response to oxidative stress, cellular homeostasis, cell signaling, and mitochondrial structure. In contrast, in mice with targeted disruption of Dio2 in astrocytes (Astro D2KO mouse), the highly induced genes in the hippocampus were related to inflammation, apoptosis, and cell death. We propose that Dio2 induction caused by SE accelerates production of T3 in different areas of the central nervous system and modifies the hippocampal gene expression profile, affecting the balance between adaptive and maladaptive mechanisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|