Human polyomavirus 6 DNA in the cerebrospinal fluid of an HIV-positive patient with leukoencephalopathy
| Autor: | Sonia Villani, Eric Delwart, Serena Delbue, R. Bella, Enrico Marchioni, Tung Gia Phan, Lucia Signorini, Francesca Elia, Pasquale Ferrante |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Human cytomegalovirus viruses HIV Infections Real-Time Polymerase Chain Reaction medicine.disease_cause Leukoencephalopathy Leukoencephalopathies Virology medicine Humans Human polyomavirus 6 Cerebrospinal Fluid Polyomavirus Infections biology Progressive multifocal leukoencephalopathy Varicella zoster virus virus diseases Sequence Analysis DNA Middle Aged biology.organism_classification medicine.disease Epstein–Barr virus Infectious Diseases Lytic cycle DNA Viral Immunology Female Human herpesvirus 6 Polyomavirus |
| Zdroj: | Journal of Clinical Virology. 68:24-27 |
| ISSN: | 1386-6532 |
| Popis: | Background Leukoencephalopathies in HAART-treated, HIV-positive patients include progressive multifocal leukoencephalopathy (PML), a result of lytic infection oligodendrocytes by JC polyomavirus (JCV), and another form characterized by the absence of JCV genome in cerebrospinal fluid (CSF). Objectives To test the potential viral etiology of JCV-negative leukoencephalopathy. Study design CSF was collected from 43 HIV-positive patients with MRI suggestive of leukoencephalopathies. DNA was isolated and real-time PCR assays for neurotropic viruses (Herpes Simplex Viruses 1/2, Varicella Zoster Virus, Epstein Barr Virus, Human Cytomegalovirus, Human Herpesvirus 6, JCV and HIV) were conducted. CSF from 14 non-reactive cases were subjected to random nucleic acid amplification, deep sequencing, and in silico search for viral sequences. Results JCV genome was detected in the CSF of 19/43 PML patients, HIV genome in the CSF of 5 PML patients including 2 JCV negative patients, and no viruses were detected in 22 patients. Human Polyomavirus 6 (HPyV6) DNA was detected by deep sequencing in one JCV-negative leukoencephalopathy CSF sample. Conclusions HPyV6 DNA was detected in CSF of a case of demyelinating disease. HPyV6 has not been previously reported in CSF or associated with any disease. Demonstrating a causative role will require further studies. |
| Databáze: | OpenAIRE |
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