Effects of Recombinant Human AMH on SCF Expression in Human Granulosa Cells
| Autor: | Rong Hu, Xiao-Mei Zhang, Xing Wu, Fei-Miao Wang, Ying-Pu Sun, Juan Li, Yan Lou, Hui-Ming Ma |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Anti-Mullerian Hormone medicine.medical_specialty Biophysics Stem cell factor Biology Immunofluorescence Biochemistry law.invention Andrology law Internal medicine medicine Humans RNA Messenger Cells Cultured Stem Cell Factor Messenger RNA Granulosa Cells medicine.diagnostic_test RNA Anti-Müllerian hormone Cell Biology General Medicine Recombinant Proteins Endocrinology embryonic structures Recombinant DNA biology.protein Matched group Female Hormone |
| Zdroj: | Cell Biochemistry and Biophysics. 67:1481-1485 |
| ISSN: | 1559-0283 1085-9195 |
| Popis: | The aim of the present study was to investigate the effects of recombinant human anti-mullerian hormone (rhAMH) on Stem Cell Factor (SCF) expression in human granulosa cells (GCs). GCs were obtained from infertile patients undergoing IVF-ET cycles and cultured with 20 ng/ml of rhAMH. The levels of SCF mRNA and protein were detected in both matched and experimental group by real-time PCR, immunofluorescence, and ELISA, respectively, on day 4 of culture. We found that human GCs expressed SCF mRNA and protein, and SCF expression in the experimental group was significantly lower than that in the matched group (p < 0.05). We further showed that rhAMH inhibited SCF expression at mRNA and protein levels. |
| Databáze: | OpenAIRE |
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