Transcriptional squelching re-examined
| Autor: | Elizabeth Molinari, Sridaran Natesan, Victor M. Rivera, Michael Gilman |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cell signaling
Multidisciplinary Transcription Genetic General transcription factor Recombinant Fusion Proteins Computational biology Biology Alkaline Phosphatase Transfection Cell biology Chromatin Transcriptome Gene Expression Regulation Genes Reporter Transcription (biology) Tumor Cells Cultured Humans Signal transduction Functional genomics Gene Plasmids Transcription Factors |
| Zdroj: | Nature. 390:349-350 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/37019 |
| Popis: | The introduction of a potent transcriptional activator into eukaryotic cells can paradoxically suppress the transcription of a co-introduced target gene1,2,3. This so-called ‘squelching’ is thought to result from titration of one or more general transcription factors (GTFs)4, indicating that GTFs might be in limited supply within the cell5,6. We now find that in mammalian cells, squelching is limited to episomal target genes, whereas genes integrated into cellular chromatin are immune. |
| Databáze: | OpenAIRE |
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