Venezuelan equine encephalomyelitis virus: Concentration, partial purification, inactivation and immunogenicity
| Autor: | T E Walton, T L Barber, N M Foster |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Encephalomyelitis
Equine viruses Encephalomyelitis Guinea Pigs Immunology Hemagglutinins Viral Virulence Centrifugation Isopycnic Antibodies Viral Vaccines Attenuated complex mixtures Microbiology Virus Encephalitis Virus Venezuelan Equine medicine Animals Immunology and Allergy Centrifugation Horses Infectivity General Veterinary biology Immunogenicity Encephalomyelitis Venezuelan Equine Viral Vaccines General Medicine medicine.disease Virology Infectious Diseases Inactivated vaccine biology.protein Horse Diseases Antibody |
| Zdroj: | Comparative Immunology, Microbiology and Infectious Diseases. 6:31-37 |
| ISSN: | 0147-9571 |
| DOI: | 10.1016/0147-9571(83)90034-6 |
| Popis: | Venezuelan equine encephalomyelitis (VEE) TC-84 vaccinal virus, from 10-1. quantities of infected duck embryo fibroblast cell culture fluids, was isolated by combined continuous-flow centrifugation with isopycnic banding in sucrose. Most of the recovered infectivity and hemagglutinating activity were in a single band at a buoyant density (rho) of 1.2. About 90% of the total input protein (450-520 mg) was removed with the effluent, whereas most of the remaining 10% also banded at a rho of 1.2. Infectivity was inactivated with formalin at a final concentration of 0.05% at 37 degrees C for 24 hr. Formalin-inactivated virus retained its immunogenicity and induced VEE virus-specific antibody in horses and guinea pigs. The horses and those guinea pigs that received equivalent doses of vaccine survived after a challenge of their immunity with virulent VEE virus. |
| Databáze: | OpenAIRE |
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