The in vitro assessment of a novel vaping technology
| Autor: | Tomasz Jaunky, Anya Terry, Damien Breheny, Mark Taylor, S. Bozhilova, David Thorne, Marianna Gaça, Andrew Baxter, Simone Santopietro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
TPM
total particulate matter In vitro test Health Toxicology and Mutagenesis RWD relative wound density 010501 environmental sciences Pharmacology DMSO dimethyl sulfoxide Toxicology 01 natural sciences NHBE normal human bronchial epithelial ACM aerosol collected mass chemistry.chemical_compound 0302 clinical medicine HCI Health Canada Intense HUVEC human umbilical vein endothelial cell NRU neutral red uptake NASEM US National Academy of Sciences Engineering and Medicine Electronic cigarette Cigarette ANOVA analysis of variance Chemistry FDA US Food and Drug Administration In vitro toxicology ALI air-liquid interface AqE aerosol aqueous extract Regular Article MLA mouse lymphoma assay DCF 2′ 7′ dichlorodihydrofluorescein NVP new vapour product GEF global evaluation factor MF mutant frequency S9 post-mitochondrial supernatant TPA 12-O-tetradecanoylphorbol-13-acetate ARE antioxidant response element ISO International Organisation for Standardisation GSH glutathione (reduced form) 03 medical and health sciences In vitro lcsh:RA1190-1270 IVMn in vitro micronucleus DSB double-strand break Aerosol 0105 earth and related environmental sciences ComputingMethodologies_COMPUTERGRAPHICS lcsh:Toxicology. Poisons TobReg WHO Study Group on Tobacco Product Regulation WA whole aerosol CRM81 CORESTA recommended method number 81 AqE aqueous aerosol extracts 030217 neurology & neurosurgery Toxicant |
| Zdroj: | Toxicology Reports, Vol 7, Iss, Pp 1145-1156 (2020) Toxicology Reports |
| ISSN: | 2214-7500 |
| Popis: | Graphical abstract We have developed a novel vaping product (NVP) IS1.0(TT), which utilises a stainless-steel mesh to transfer and vaporise the e-liquid, mitigating some of the potential sources of toxicants that can be generated using the more traditional ‘wick and coil’ approach. The emissions from IS1.0(TT) have previously been found to have lower levels of toxicants overall when directly compared with a commercial wick and coil e-cig. This current study assessed the toxicological responses to aerosols from this NVP. Responses induced by IS1.0(TT)were compared to those from a 3R4F reference cigarette, using in vitro test methods which included regulatory genetic toxicological assays as well as some more contemporary screening approaches. The experimental conditions were designed to facilitate the testing of aerosol from this vaping product at doses that in most cases greatly exceeded those of the 3R4F comparator showed little to no toxicological responses and demonstrated significantly reduced effects in these in vitro assays when compared to 3R4F. Furthermore, the extreme doses tested in the present study indicate that the toxicant profile of this NVP translates to lower biological activity in vitro, and suggests that the absolute risk hazard level associated with electronic cigarettes can be reduced through continuous improvement as the technology evolves. |
| Databáze: | OpenAIRE |
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