A Rho-actin signaling pathway shapes cell wall boundaries in Arabidopsis xylem vessels
| Autor: | Mayuko Sato, Kiminori Toyooka, Mayumi Wakazaki, Yoshinobu Nagashima, Hiroo Fukuda, Yuki Sugiyama, Yoshihisa Oda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
rho GTP-Binding Proteins Science Cell Arabidopsis General Physics and Astronomy 02 engineering and technology Microtubules General Biochemistry Genetics and Molecular Biology Article Cell wall Cell membrane 03 medical and health sciences Microtubule Cell Wall Gene Expression Regulation Plant Xylem medicine lcsh:Science Cell Shape Actin Cells Cultured Multidisciplinary Microscopy Confocal biology Effector Chemistry Arabidopsis Proteins Cell Membrane General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification Plants Genetically Modified eye diseases Actins Cell biology Luminescent Proteins 030104 developmental biology medicine.anatomical_structure lcsh:Q Signal transduction 0210 nano-technology Signal Transduction |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-10 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Patterned cell wall deposition is crucial for cell shapes and functions. In Arabidopsis xylem vessels, ROP11 GTPase locally inhibits cell wall deposition through microtubule disassembly, inducing pits in cell walls. Here, we show that an additional ROP signaling pathway promotes cell wall growth at pit boundaries. Two proteins, Boundary of ROP domain1 (BDR1) and Wallin (WAL), localize to pit boundaries and regulate cell wall growth. WAL interacts with F-actin and promotes actin assembly at pit boundaries while BDR1 is a ROP effector. BDR1 interacts with WAL, suggesting that WAL could be recruited to the plasma membrane by a ROP-dependent mechanism. These results demonstrate that BDR1 and WAL mediate a ROP-actin pathway that shapes pit boundaries. The study reveals a distinct machinery in which two closely associated ROP pathways oppositely regulate cell wall deposition patterns for the establishment of tiny but highly specialized cell wall domains. Cell wall pits allow movement of water between xylem vessels and are formed via Rho-GTPase mediated signaling that leads to local microtubule disassembly. Here, Sugiyama et al. show that an additional Rho-GTPase pathway controls cell wall deposition and actin dynamics to form pit boundaries. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|