Production of specific polyclonal antibodies anti-human factor IX
| Autor: | Wanderley Almeida Ferreira, Daniel Gonçalves Chaves, Cibele V. Rodrigues, Clara Guerra Duarte, Marcelo M. Santoro, Anna Bárbara F. Carneiro-Proietti, Pollyana Fantini Miranda Siqueira |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
business.operation
purification lcsh:Biotechnology Octapharma Factor IX Antigen lcsh:TP248.13-248.65 medicine antibodies Bovine serum albumin chemistry.chemical_classification Multidisciplinary biology Chemistry Trypsin Molecular biology Enzyme Polyclonal antibodies biology.protein hemophilia B ELISA Antibody HPLC business medicine.drug |
| Zdroj: | Brazilian Archives of Biology and Technology v.49 n.4 2006 Brazilian Archives of Biology and Technology Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR Brazilian Archives of Biology and Technology, Volume: 49, Issue: 4, Pages: 605-609, Published: JUL 2006 Brazilian Archives of Biology and Technology, Vol 49, Iss 4, Pp 605-609 (2006) |
| Popis: | In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.Neste trabalho foram produzidos anticorpos policlonais anti-fator IX humano em coelhos New Zealand imunizados com FIX humano (hFIX) comercial puro (Octanyne®, Octapharma, EUA). O soro contendo as imunoglobulinas anti-hFIX foi útil para a detecção do antígeno hFIX em frações do plasma humano submetido a cromatografia de troca iônica. Imunoensaios (ELISA) usando soro-albumina bovina, tripsina e peptídeos gerados por ensaios de clivagem com tripsina com enzima de digestão foram realizados e revelaram especificidade adequada dos anticorpos policlonais produzidos. |
| Databáze: | OpenAIRE |
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