Comparison of adsorption and conjugation of Herceptin on poly(lactic-co-glycolic acid) nanoparticles - Effect on cell internalization in breast cancer cells
| Autor: | Woo Suk Jang, Jin-Seok Choi, Jeong-Sook Park |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
media_common.quotation_subject Cell Nanoparticle Bioengineering Breast Neoplasms 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound Adsorption Pulmonary surfactant Polylactic Acid-Polyglycolic Acid Copolymer medicine Humans Internalization Cytotoxicity Glycolic acid media_common Trastuzumab 021001 nanoscience & nanotechnology 0104 chemical sciences medicine.anatomical_structure chemistry Mechanics of Materials Biophysics MCF-7 Cells Surface modification Nanoparticles Female 0210 nano-technology |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 92 |
| ISSN: | 1873-0191 |
| Popis: | Surfaces of nanoparticles with are commonly modified to enhance the targeting effect. In this study, we performed surface modifications of docetaxel (DTX)-loaded poly(lactic-co-glycolic acid) nanoparticles (PNPs) with Herceptin® (HCT) to improve the internalization and cytotoxicity in breast cancer cells. The PNPs were prepared with surfactant, poly(ethylene-alt-maleic anhydride) (PEMA), including a number of carboxyl groups for conjugation. Three types of PNPs were prepared via different methods such as adsorption, charged adsorption, and bio-conjugation. The PNPs were evaluated in terms of physical properties, stability, cellular uptake and cytotoxicity. The docetaxel-loaded PNPs with HCT were successfully surface-modified with mean particle sizes of 338.4 ± 59.8 nm (DTX-PNPs), 353.9 ± 67.5 nm (HCT-A-DTX-PNPs), 544.8 ± 301.7 nm (HCT-C-DTX-PNPs), and 499.1 ± 71.9 nm (HCT-B-DTX-PNPs). Cellular uptake of HCT-B-PNPs was 5.0-, 4.4-, and 4.6-fold higher than that of PNPs in BT-474, SK-BR-3, and MCF-7 cells, respectively, at 2 h. At 40 μg/mL, HCT-B-DTX-PNPs showed a higher cytotoxicity toward BT-474, SK-BR-3, and MCF-7 cells than the other formulations. In conclusion, HCT-B-DTX-PNPs were found to possess a higher affinity for breast cancer cells and induce a stronger cytotoxicity than that of other PNPs. |
| Databáze: | OpenAIRE |
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