Immunological Responses to Seoul Orthohantavirus in Experimentally and Naturally Infected Brown Rats (Rattus norvegicus)
| Autor: | Sithumini M. W. Lokupathirage, Devinda S. Muthusinghe, Jiro Arikawa, Takaaki Koma, Futoshi Hasebe, Mai Quynh Le, Zhuoxing Wei, Kumiko Yoshimatsu, Tetsu Yamashiro, Shumpei P. Yasuda, Kenta Shimizu, Nguyen Thuy Hoa |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male reservoir IgM lcsh:QR1-502 chemical and pharmacologic phenomena bunyavirus Biology Antibodies Viral Article lcsh:Microbiology Immunoglobulin G CTLs 03 medical and health sciences 0302 clinical medicine Immune system Virology hemorrhagic fever with renal syndrome Cytotoxic T cell Animals 030212 general & internal medicine Seoul virus Viral Load Rats Chronic infection CTL 030104 developmental biology Infectious Diseases Immunoglobulin M biology.protein Female Antibody Viral load |
| Zdroj: | Viruses Volume 13 Issue 4 Viruses, Vol 13, Iss 665, p 665 (2021) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v13040665 |
| Popis: | To clarify the mechanism of Seoul orthohantavirus (SEOV) persistence, we compared the humoral and cell-mediated immune responses to SEOV in experimentally and naturally infected brown rats. Rats that were experimentally infected by the intraperitoneal route showed transient immunoglobulin M (IgM) production, followed by an increased anti-SEOV immunoglobulin G (IgG) antibody response and maturation of IgG avidity. The level of SEOV-specific cytotoxic T lymphocytes (CTLs) peaked at 6 days after inoculation and the viral genome disappeared from serum. In contrast, naturally infected brown rats simultaneously had a high rate of SEOV-specific IgM and IgG antibodies (28/43). Most of the IgM-positive rats (24/27) had the SEOV genome in their lungs, suggesting that chronic SEOV infection was established in those rats. In female rats with IgG avidity maturation, the viral load in the lungs was decreased. On the other hand, there was no relationship between IgG avidity and viral load in the lungs in male rats. A CTL response was not detected in naturally infected rats. The difference between immune responses in the experimentally and naturally infected rats is associated with the establishment of chronic infection in natural hosts. |
| Databáze: | OpenAIRE |
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