Cloning and Immunosuppressive Properties of an Acyl-Activating Enzyme from the Venom Apparatus of Tetrastichus brontispae (Hymenoptera: Eulophidae)
| Autor: | Youming Hou, Bao-Zhen Tang, Xiao-Mei Zhang, Cheng-Jun Ma, Min Liu, Hua-Jian Zhang, Ting-Ting Fu, Bin Liu, Xia-Fang Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Health Toxicology and Mutagenesis lcsh:Medicine Venom Toxicology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 4-coumarate:coa ligase-like 4 acyl-activating enzyme Peptide sequence 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Fatty acid metabolism Polydnavirus lcsh:R parasitoid venom Fatty acid parasitization biology.organism_classification immunity Amino acid 010602 entomology Enzyme chemistry Biochemistry host octodonta nipae parasitoid wasp tetrastichus brontispae Tetrastichus |
| Zdroj: | Toxins, Vol 11, Iss 11, p 672 (2019) Toxins Volume 11 Issue 11 |
| ISSN: | 2072-6651 |
| Popis: | Venom injected into the host plays vital roles in facilitating successful parasitization and development for parasitoid wasps, especially those devoid of polydnavirus, and the abundant venom proteins appear to be most likely involved in parasitization success. Previously, we found the four most abundant venom proteins, including 4-coumarate:CoA ligase-like 4 (4CL4-like), in the Tetrastichus brontispae (Hymenoptera: Eulophidae) venom apparatus. In this study, we cloned, expressed T. brontispae 4CL4-like (Tb4CL4-like) in Escherichia coli, and investigated its immunosuppressive properties. The deduced amino acid sequence for Tb4CL4-like shares high identity at conserved amino acids associated with the acyl-activating enzyme (AAE) consensus motif but shows only < 40% identity with the members in the AAE superfamily. mRNA abundance analysis indicated that Tb4CL4-like was transcribed mainly in the venom apparatus. Recombinant Tb4CL4-like inhibited Octodonta nipae (Coleoptera: Chrysomelidae) pupal cellular encapsulation and spreading by targeting the hemocyte cytoskeleton and reduced the hemocyte-mediated phagocytosis of E. coli in vivo. Moreover, Tb4CL4-like exhibited greater affinity to palmitic acid and linolenic acid based on the molecular docking assay and is hypothesized to be involved in fatty acid metabolism. In conclusion, our results suggest that Tb4CL4-like may be an immunity-related AAE protein that is involved in the regulation of host immunity through fatty acid metabolism-derived signaling pathways. |
| Databáze: | OpenAIRE |
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