The best approach for early prediction of fetal gender by using free fetal DNA from maternal plasma
| Autor: | Alessia Fanetti, Luana Pennacchi, Michela Centra, Giuliana Coata, Gian Carlo Di Renzo, Elena Picchiassi |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Sex Determination Analysis medicine.medical_specialty DYS14 Fetal gender Noninvasive prenatal diagnosis Real-time PCR SRY Prenatal diagnosis Polymerase Chain Reaction Sensitivity and Specificity Plasma Predictive Value of Tests Pregnancy Prenatal Diagnosis medicine Humans Neonatology Maternal-Fetal Exchange Genetics (clinical) Gynecology Fetus Obstetrics business.industry Obstetrics and Gynecology DNA medicine.disease Real-time polymerase chain reaction Testis determining factor Cell-free fetal DNA Gestation Female business |
| Zdroj: | Prenatal Diagnosis. 28:525-530 |
| ISSN: | 1097-0223 0197-3851 |
| DOI: | 10.1002/pd.2018 |
| Popis: | Objectives Detection of free fetal DNA (ffDNA) in maternal blood during pregnancy has given rise to the possibility of developing new noninvasive approaches for early prenatal diagnosis. On a large-scale study, two protocols of real-time polymerase chain reaction (PCR) were compared in order to establish which Y-specific locus, either multicopy DYS14 or single copy SRY sequence, was the most suitable for developing a test with high diagnostic efficiency for early fetal gender assessment. The second aim was to assess whether the combination of the two detection systems could increase the performance of the prenatal test. Methods We analyzed 145 plasma samples from healthy pregnant women between 11 and 12 weeks of singleton gestation. For each sample, fetal gender was determined by using both protocols (DYS14 and SRY ) during the same real-time PCR run. Results The data obtained by the DYS14 and SRY assays showed an efficiency in fetal gender prediction of 97.9 and 80%, respectively. It is not advisable to combine the two protocols because this association does not help in further improvements in fetal gender prediction. Conclusions DYS14 assay is the best approach for early fetal gender assessment because it is more sensitive, accurate, and efficient than the SRY assay. Copyright 2008 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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