Curcumin attenuates migration of vascular smooth muscle cells via inhibiting NFκB-mediated NLRP3 expression in spontaneously hypertensive rats
| Autor: | Yun Qiu, Qi Chen, Ai-Dong Chen, Yu-Ming Kang, Ying Tong, Yun-Zhi Chen, Feng Zhang, Ying Han, Hai-Jian Sun, Yuehua Li, Guo-Qing Zhu, Xiao-Hong Qi, Chao Ye |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Vascular smooth muscle Curcumin Endocrinology Diabetes and Metabolism Clinical Biochemistry Cell Inflammation Blood Pressure 030204 cardiovascular system & hematology Pharmacology Biochemistry Muscle Smooth Vascular 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Movement Heart Rate Rats Inbred SHR Renin–angiotensin system NLR Family Pyrin Domain-Containing 3 Protein medicine Animals Rats Wistar Receptor Molecular Biology Aorta Cells Cultured Gene knockdown Nutrition and Dietetics Dose-Response Relationship Drug Chemistry Angiotensin II NF-kappa B Interleukin musculoskeletal system 030104 developmental biology medicine.anatomical_structure Hypertension cardiovascular system medicine.symptom tissues |
| Zdroj: | The Journal of nutritional biochemistry. 72 |
| ISSN: | 1873-4847 |
| Popis: | Migration of vascular smooth muscle cell (VSMC) plays a critical role in the pathophysiology of hypertension and several other vascular diseases. Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione), a bioactive constituent from Curcuma longa, is commonly used as a spice, food additive or dietary pigment. It has several health benefits including antioxidant, anti-inflammatory and anticancer properties. This study examined the roles of curcumin in VSMC migration in hypertension and underlying mechanism. VSMC was isolated and prepared from thoracic aorta of Wistar-Kyoto rats and spontaneously hypertensive rats (SHR). VSMC migration was evaluated with Boyden chamber assay and wound-healing assay. Curcumin attenuated VSMC migration, inhibited nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) expression and reduced interleukin (IL)-1β concentration in VSMC of SHR, which were similar to the effects of NLRP3 knockdown on IL-1β concentration and VSMC migration. Curcumin inhibited NFκB activation in VSMC of SHR, which was similar to the effects of NFκB inhibitor BAY11-7082 on NFκB activation. In another in vitro model of rat VSMC migration, curcumin also inhibited angiotensin II-induced VSMC migration, NFκB activation, NLRP3 expression and IL-1β production. Intragastric administration of curcumin in SHR attenuated hypertension and reduced NFκB activation, NLRP3 and matrix metalloproteinase-9 expressions and aortic media thickness. These results indicate that curcumin inhibits VSMC migration via inhibiting NFκB-mediated NLRP3 expression in VSMC of SHR or in angiotensin II-treated VSMC. Curcumin attenuates hypertension, vascular inflammation and vascular remodeling in SHR. |
| Databáze: | OpenAIRE |
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