Transcriptional Regulation of the α-fetoprotein Gene by SWI/SNF Chromatin Remodeling Complex

Autor:	Katsuhide Miyake, Hidenori Kaneoka, Shinji Iijima, Masamichi Kamihira, Mikio Takahashi, Yoshitaka Sato, Takeaki Dohda, Yujin Inayoshi
Rok vydání:	2005
Předmět:	Ccaat-enhancer-binding proteins Clinical Biochemistry Biomedical Engineering Bioengineering Cell Biology Biology Molecular biology Article Chromatin remodeling SWI/SNF Chromatin Transactivation embryonic structures Coactivator Transcriptional regulation Transcription factor Biotechnology
Zdroj:	Cytotechnology. 49:143-151
ISSN:	1573-0778 0920-9069
DOI:	10.1007/s10616-006-6702-2
Popis:	α-Fetoprotein (AFP) is a fetal serum protein abundant in fetal liver whose expression is downregulated in adult liver. The promoter–proximal region of the AFP gene contains two binding sites for CCAAT/enhancer-binding protein α (C/EBPα), two for hepatocyte nuclear factor-1α (HNF-1α) and one for nuclear factor-1 (NF-1). Luciferase reporter assays showed that a combination of C/EBPα, HNF-1α, NF-1 and coactivator p300 gave the maximal activity but the BRG1 and BRM, ATPase subunit of the chromatin remodeling complex SWI/SNF, repressed the transactivation of the AFP gene by these transcription factors. Deletion analyses of C/EBPα binding sites suggested that C/EBPα recruited SWI/SNF complex and caused the repression. This repression may also play important roles in the downregulation of the AFP gene in adult hepatocytes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3aa26dd9afabb6c7c9ecca5f552e910b https://doi.org/10.1007/s10616-006-6702-2 Zobrazit plný text záznamu Full text from SpringerLink