Association study of 15 novel single-nucleotide polymorphisms of the T-bet locus among Finnish asthma families
| Autor: | Riitta Lahesmaa, Maritta Pykäläinen, Riikka Kinos, Juha Kere, Lauri A. Laitinen, Johannes Savolainen, Tarja Laitinen, N. Sirkkanen, Emmi Ylikoski |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Candidate gene Linkage disequilibrium Immunology chemical and pharmacologic phenomena Locus (genetics) Single-nucleotide polymorphism Immunoglobulin E Polymorphism Single Nucleotide Linkage Disequilibrium 03 medical and health sciences 0302 clinical medicine Humans Immunology and Allergy Medicine SNP Finland 030304 developmental biology Genetics 0303 health sciences Chi-Square Distribution biology business.industry Haplotype hemic and immune systems Asthma 3. Good health biology.protein Female T-Box Domain Proteins business Transcription Factors 030215 immunology Founder effect |
| Zdroj: | Clinical Experimental Allergy. 34:1049-1055 |
| ISSN: | 1365-2222 0954-7894 |
| Popis: | ummary Objective T-box expressed in T cells (T-bet) is a transcription factor regulating the commitment of T helper (Th) cells by driving the cells into the Th1 direction. Abnormal Th1/Th2 balance may lead to complex disorders like asthma or autoimmune diseases. Recent studies have suggested that T-bet might be a candidate gene for asthma. This led us to screen 23 Finnish individuals for single-nucleotide polymorphisms (SNPs) in the T-bet locus and study the association between the SNPs and high serum IgE level and asthma. Methods We screened all six exons, adjacent intronic areas and 2 kb of the 5′-flanking region from 23 individuals utilizing WAVE™ technology. To explore whether T-bet is associated in serum IgE regulation or asthma we genotyped the SNPs in a Finnish asthmatic founder population. The association analyses were made using haplotype pattern mining. Results Fifteen novel SNPs were found in the T-bet gene. Within the Finnish asthmatic founder population, there was no association between T-bet SNPs and high serum IgE level or asthma. Conclusions The genetic variability in the T-bet gene does not play a role in the pathogenesis of human asthma. Our results provide a novel panel of SNPs in T-bet and will help determine whether the SNPs have a functional role in other T cell-mediated diseases. |
| Databáze: | OpenAIRE |
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