Cystatin C-Adiponectin Complex in Plasma Associates with Coronary Plaque Instability
| Autor: | Hiroyasu Yamamoto, Shinji Kihara, Tetsuro Matsuoka, Akane Matsumoto, Sumire Onishi, Natsumi Matsuo, Kento Kayama |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male medicine.medical_specialty animal structures Immunoprecipitation Coronary Artery Disease 030204 cardiovascular system & hematology Coronary artery disease 03 medical and health sciences Necrosis 0302 clinical medicine In vivo Internal medicine Internal Medicine medicine Humans Clinical significance 030212 general & internal medicine Cystatin C Aged Plaque Aged 80 and over biology Adiponectin business.industry Biochemistry (medical) Middle Aged medicine.disease Atherosclerosis Fibrosis Lipids In vitro Plaque Atherosclerotic Endocrinology HEK293 Cells Multiprotein Complexes Multivariate Analysis biology.protein Original Article Antibody Cardiology and Cardiovascular Medicine business hormones hormone substitutes and hormone antagonists Biomarkers |
| Zdroj: | Journal of Atherosclerosis and Thrombosis |
| ISSN: | 1880-3873 |
| Popis: | Aim Adiponectin (APN) is an adipocyte-derived bioactive molecule with antiatherogenic properties. We previously reported that cystatin C (CysC) abolished the anti-atherogenic effects of APN. We aimed to elucidate the clinical significance of CysC-APN complex in patients with coronary artery disease (CAD). Methods We enrolled 43 stable CAD male patients to examine the relationship between CysC-APN complex and coronary plaque characteristics. Serum was immunoprecipitated by the anti-APN antibody and immunoblotted by the anti-CysC antibody to demonstrate the presence of CysC-APN complexes in vivo. To confirm their binding in vitro, HEK293T cell lysates overexpressing myc-APN and FLAG-CysC were immunoprecipitated with an anti-myc or anti-FLAG antibody, followed by immunoblotting with an anti-APN or anti-CysC antibody. Results CysC was identified as a specific co-immunoprecipitant with APN by the anti-APN antibody in human serum. In vitro, FLAG-CysC was co-immunoprecipitated with myc-APN by the anti-myc antibody and myc-APN was co-immunoprecipitated with FLAG-CysC by the anti-FLAG antibody. Among CAD patients, serum CysC-APN complex levels negatively correlated with fibrotic components of coronary plaques and positively correlated with either necrotic or lipidic plus necrotic components. Plaque burden negatively correlated with serum APN levels but not serum CysC-APN complex levels. Serum CysC levels had no association with plaque characteristics. In multivariate analysis, CysC-APN complex levels were identified as the strongest negative factor for fibrotic components and the strongest positive factor for both necrotic and lipidic plus necrotic components. Conclusion Measuring serum CysC-APN complex levels is helpful for evaluating coronary plaque instability in CAD patients. |
| Databáze: | OpenAIRE |
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