Quantum dot coating of baculoviral vectors enables visualization of transduced cells and tissues
Autor: | Yuangang Zheng, Chunxiao Wu, Seong Loong Lo, Ming-Yong Han, Shu Wang, Dang Hoang Lam, Ying Zhao |
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Rok vydání: | 2013 |
Předmět: |
Transgene
Genetic enhancement Genetic Vectors Biophysics Mice Nude Gene delivery Biology Biochemistry Mice Viral envelope Transduction Genetic In vivo Cell Line Tumor Quantum Dots Animals Humans Vector (molecular biology) Molecular Biology Mice Inbred BALB C Optical Imaging Genetic Therapy Cell Biology Molecular biology Nucleopolyhedroviruses Cell biology Quantum dot Cell culture Female Protein Binding |
Zdroj: | Biochemical and Biophysical Research Communications. 434:110-116 |
ISSN: | 0006-291X |
Popis: | Imaging of transduced cells and tissues is valuable in developing gene transfer vectors and evaluating gene therapy efficacy. We report here a simple method to use bright and photostable quantum dots to label baculovirus, an emerging gene therapy vector. The labeling was achieved through the non-covalent interaction of glutathione-capped CdTe quantum dots with the virus envelope, without the use of chemical conjugation. The quantum dot labeling was nondestructive to viral transduction function and enabled the identification of baculoviral vector-transduced, living cells based on red fluorescence. When the labeled baculoviral vectors were injected intravenously or intraventricularly for in vivo delivery of a transgene into mice, quantum dot fluorescence signals allow us monitor whether or not the injected tissues were transduced. More importantly, using a dual-color whole-body imaging technology, we demonstrated that in vivo viral transduction could be evaluated in a real-time manner in living mice. Thus, our method of labeling a read-to-use gene delivery vector with quantum dots could be useful towards the improvement of vector design and will have the potential to assess baculovirus-based gene therapy protocols in future. |
Databáze: | OpenAIRE |
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