Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp

Autor:	Mariela Echeverria, Rodolfo A. Ugalde, Ana Bernardina Menéndez, Cintia Sánchez, Valeria P. Conforte, Viviana C. Lepek
Rok vydání:	2010
Předmět:	Rhizobiaceae biology Lotus japonicus Lotus Structural gene biology.organism_classification Plant Roots Applied Microbiology and Biotechnology Microbiology Rhizobia Mesorhizobium loti Symbiosis Botany Carbon-Carbon Lyases Microbial inoculant Alphaproteobacteria
Zdroj:	The Journal of General and Applied Microbiology. 56:331-338
ISSN:	1349-8037 0022-1260
DOI:	10.2323/jgam.56.331
Popis:	Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this gene is under the activity of the NifA-RpoN-dependent promoter; thus, it is only expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was integrated into the M. loti chromosome under a constitutive promoter activity. The resulting strain induced the formation of a higher number of nodules and was more competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results suggest that the introduction of the ACC deaminase activity within M. loti in a constitutive way could be a novel strategy to increase nodulation competitiveness of the bacteria, which could be useful for the forage inoculants industry.
Databáze:	OpenAIRE
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