P-Glycoprotein Is Positively Correlated with p53 Protein Accumulation in Human Colorectal Cancers
| Autor: | Junji Tsurutani, Isao Matsuo, F Narasaki, Hiroshi Soda, Katsuhisa Omagari, Koki Ikeda, Jean M. Gudas, Minoru Fukuda, Kiyoshi Kounoura, Akira Sakamoto, Mikio Oka, Yohei Mizuta, Nobuhiro Ikuno, Shigeru Kohno |
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| Rok vydání: | 1997 |
| Předmět: |
p53
Cancer Research Pathology medicine.medical_specialty Mutant Multidrug resistance Adenocarcinoma Article Gene product In vivo Gene expression P‐glycoprotein polycyclic compounds medicine Humans ATP Binding Cassette Transporter Subfamily B Member 1 Promoter Regions Genetic P-glycoprotein Frozen section procedure integumentary system biology Genes p53 medicine.disease Molecular biology Colon cancer Neoplasm Proteins Gene Expression Regulation Neoplastic Oncology biology.protein Genes MDR Tumor Suppressor Protein p53 Antibody Colorectal Neoplasms MDR1 gene |
| Zdroj: | Japanese Journal of Cancer Research : Gann |
| ISSN: | 0910-5050 |
| DOI: | 10.1111/j.1349-7006.1997.tb00445.x |
| Popis: | To explore the relationship between mutant p53 and Pgp expression, we have examined the levels of both proteins in human colorectal adenocarcinomas. Serial frozen sections of 40 surgical samples were stained with an anti-Pgp (MRK16) and two different anti-p53 protein antibodies (Abs), PAb421 and PAb1801. Nineteen (47.5%) of 40 samples examined were positive for Pgp, and 18 (45%) of 40 were positive for p53. The samples that stained positively with PAb421 also stained positively with PAb1801. Pgp expression was detected in 13 (76.5%) of 17 samples that were positive for p53 using PAb421 and in 15 (83.3%) of 18 samples that were positive for p53 using PAb1801. Thus, we found that p53 and Pgp were co-expressed in a significant number of samples (P < 0.002). There was no relationship between Pgp or p53 protein accumulation and histologic grade or stage. The present results demonstrate that Pgp expression is closely associated with p53 protein accumulation in human colorectal cancers. These data provide evidence to support the idea that mutant p53 activates the MDR1 gene in vivo. |
| Databáze: | OpenAIRE |
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