Protective Effect of Grape and Apple Juices against Cadmium Intoxication in the Kidney of Rats
| Autor: | Luciana Pellegrini Pisani, Bianca Andrade Handan, Caroline Margonato Cardoso, Aline Boveto Santamarina, Daniel Araki Ribeiro, Carolina Foot Gomes de Moura |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
inorganic chemicals Cadmium Poisoning Necrosis Antioxidant medicine.medical_treatment Intraperitoneal injection chemistry.chemical_element 010501 environmental sciences Cadmium chloride Pharmacology Kidney Protective Agents 01 natural sciences Antioxidants 03 medical and health sciences chemistry.chemical_compound Cadmium Chloride Drug Discovery medicine Animals Humans Regeneration Vitis Rats Wistar Saline 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Cadmium fungi General Medicine Rats Fruit and Vegetable Juices Disease Models Animal Oxidative Stress medicine.anatomical_structure chemistry Malus Toxicity Environmental Pollutants medicine.symptom Injections Intraperitoneal DNA Damage |
| Zdroj: | Drug Research. 70:503-511 |
| ISSN: | 2194-9387 2194-9379 |
| DOI: | 10.1055/a-1221-4733 |
| Popis: | Background The objective of this study was to evaluate protective effect of grape and apple juices against toxicity induced by cadmium in the kidney of rats. Methods A total of 20 male-Wistar rats were distributed into four groups (n=5): Control group: animals received an intraperitoneal (i.p.) injection of 0.9% saline solution and after 15 days, 1 mL of water was administered for 15 days, via gavage; Cadmium group: animals received an intraperitoneal injection of cadmium chloride (1.2 mg/kg) and after 15 days, 1 mL of water was administered for 15 days via gavage; Cadmium+Grape Juice: animals received an i.p. injection of cadmium chloride (1.2 mg/kg), and after 15 days, 0.8 mL of grape juice was administered for 15 days, via gavage; Cadmium+Apple Juice: animals received i.p. injection of cadmium chloride (1.2 mg/kg) and after 15 days, 1.0 mL of apple juice was administered for 15 days, via gavage. Results Histopathological analysis revealed severe tubular lesion and necrosis in the group exposed to cadmium, while animals exposed to grape or apple juices showed a significant reduction of tissue injury. 8-OHdG immunoexpression, DNA damage, cytochrome C and catalase gene expressions and Toll like signaling pathway (TLR2, and pIKKα/β) decreased in animals treated with grape juice when compared to cadmium group. Conclusion Taken together, we conclude that grape and apple juices had a protective effect by means of antioxidant, antigenotoxic actions and for promoting tissue regeneration in the kidney of rats following cadmium intoxication. |
| Databáze: | OpenAIRE |
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