Laboratory and Wild-Type Klebsiella pneumoniae Strains Carrying Mannose-Inhibitable Adhesins and Receptors for Coliphages T3 and T7 Are More Pathogenic for Mice Than Are Strains Without Such Receptors
Autor: | Carla Pruzzo, Sebastiano Valisena, Giuseppe Satta |
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Rok vydání: | 1983 |
Předmět: |
Lipopolysaccharides
Male Pathogenic Mechanisms Blood Bactericidal Activity Klebsiella Lipopolysaccharide Klebsiella pneumoniae Immunology Microbiology Mice chemistry.chemical_compound Bacterial Proteins Lysogenic cycle Animals Receptor biology Immunization Passive Adhesiveness Membrane Proteins biology.organism_classification Bacterial adhesin Infectious Diseases chemistry Membrane protein Receptors Virus T-Phages Parasitology Peritoneum Bacterial outer membrane Bacterial Outer Membrane Proteins |
Zdroj: | Infection and Immunity. 39:520-527 |
ISSN: | 1098-5522 0019-9567 |
DOI: | 10.1128/iai.39.2.520-527.1983 |
Popis: | We have shown previously that Klebsiella pneumoniae receptors for coliphages T3 and T7 also mediate mannose-inhibitable adherence to human epithelial cells and protect bacteria from phagocytosis and intracellular killing by human polymorphonuclear cells. In this paper we analyze the possible role of such mannose-inhibitable adhesins and T3-T7 receptors (MIAT) in K. pneumoniae intraperitoneal pathogenicity for mice. We showed that intraperitoneal pathogenicity for mice of four different Klebsiella strains (one laboratory and three wild-type) that carry the MIAT was approximately 60-fold higher than that of four derivative strains that lost such receptors by spontaneous mutation. The MIAT could be repressed by Klebsiella phage AP3 lysogenic conversion. Two laboratory and two wild-type strains converted by phage AP3 were also approximately 60-fold less pathogenic for mice than parental strains and showed a pathogenicity level equal to that of the MIAT-negative mutants. Studies of protection in mice with anti-whole cell antisera showed that passive immunization against MIAT-positive cells was more protective than immunization against MIAT-negative cells. Studies of protection in mice by both active and passive immunization with lipopolysaccharide and purified outer membrane proteins have shown that the proteins are the most protective outer membrane components. Since it has been shown previously that the Klebsiella receptors for T3-T7 have a proteic component and that an outer membrane protein is missing in the strains resistant to T3-T7 (C. Pruzzo et al., in R. C. Berkely (ed.), Microbial Adhesion to Surfaces , 1980); the latter finding further supports the role of MIAT in the pathogenicity of Klebsiella for mice. |
Databáze: | OpenAIRE |
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