T-cell heterogeneity in muscle lesions of inclusion body myositis
| Autor: | Reinhard Hohlfeld, Anke Bender, Lüder Behrens, Andrew G. Engel |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Pathology
medicine.medical_specialty Sequence analysis T-Lymphocytes T cell Immunology Population Receptors Antigen T-Cell Biology medicine.disease_cause Myositis Inclusion Body Autoimmunity medicine Humans Immunology and Allergy education In Situ Hybridization education.field_of_study Muscles T-cell receptor Antibodies Monoclonal medicine.disease Immunohistochemistry medicine.anatomical_structure Neurology Genes T-Cell Receptor beta Neurology (clinical) Inclusion body myositis CD8 |
| Zdroj: | Journal of Neuroimmunology. 84:86-91 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/s0165-5728(97)00246-4 |
| Popis: | In the muscle lesions of inclusion body myositis (IBM), two populations of T cells can morphologically be distinguished, T cells that invade muscle fibers and T cells that remain in interstitial areas. Using combined immunohistochemistry and RT-PCR, we analysed the TCR expressed by these distinct T cell populations. In three of eight IBM muscle specimens, the autoinvasive T cells were stained with one of the available mAbs: anti-Vbeta5.3 in patient 1, anti-Vbeta5.2 in patient 2, and anti-Vbeta3 in patient 3. The corresponding TCR Vbeta mRNAs were amplified by RT-PCR and the PCR products were cloned and sequenced. In all three specimens, the TCRs expressed by the autoinvasive T cells were clonally restricted. Furthermore, in patient 1 two different mAbs labelled two distinct populations of T cells: an autoaggressive population of CD8 + Vbeta5.3 + cells that invaded muscle fibers and a noninvasive interstitial population of CD8 - Vbeta5.1 + cells. TCR sequence analysis revealed that the noninvasive Vbeta5.1 + T cells were clonally diverse, whereas the autoinvasive Vbeta5.3 + T cells were clonally restricted. |
| Databáze: | OpenAIRE |
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