Activation of phenylpropanoid metabolism in sesame by over-expression of carrot calmodulin gene
| Autor: | Tetsuya Asamizu, Eriko Ishigaki, Kyoji Yamada, Fumiya Kurosaki, Ryuji Sugiyama, Satoru Mitsuma |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Transgene
Pharmaceutical Science Phenylalanine ammonia-lyase Genetically modified crops Nitric Oxide Sesamum chemistry.chemical_compound Calmodulin Caffeic acid Transgenes Phenylalanine Ammonia-Lyase Pharmacology biology Phenylpropanoid Phenylpropionates Reverse Transcriptase Polymerase Chain Reaction food and beverages General Medicine Methyltransferases biology.organism_classification Plants Genetically Modified Elicitor Daucus carota Transformation (genetics) Blotting Southern Biochemistry chemistry Agrobacterium tumefaciens Seeds Cauliflower mosaic virus |
| Zdroj: | Biologicalpharmaceutical bulletin. 27(10) |
| ISSN: | 0918-6158 |
| Popis: | Transgenic sesame (Sesamum schinzianum ASCH.) was produced by Agrobacterium-mediated transfection of a carrot calmodulin gene, cam-4, which was specifically expressed upon the contact of carrot cells with oligogalacturonide elicitor. Coding region of cam-4 was ligated to the downstream of 35S promoter of cauliflower mosaic virus and subcloned into pMATGBO-DB3.1. A. tumefaciens 4404 was transformed with the constructed vector, and the crown gall tissues formed in the sesame seedlings were transferred onto appropriate media to obtain the re-differentiated plants. The reverse-transcription polymerase chain reaction followed by Southern blot analysis revealed that cam-4 gene was appreciably expressed in the transgenic plants. Activities of two key enzyme regulating phenylpropanoid metabolisms, phenylalanine ammonia-lyase and caffeic acid O-methyltransferase, and the contents of phenolic compounds in the transformed sesame were markedly elevated as compared with those of the control. These results suggest that the over-expression of cam-4 gene enhances the biosynthetic activities of phenylpropane derivatives in the transformed sesame plants. |
| Databáze: | OpenAIRE |
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