Effect of xyloglucan (tamarind seed polysaccharide) on conjunctival cell adhesion to laminin and on corneal epithelium wound healing
| Autor: | Grazia Banchelli, Laura Raimondi, E Boldrini, Renato Pirisino, Mf Saettone, Susi Burgalassi |
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| Předmět: |
0301 basic medicine
Male Human conjunctival cells Cell adhesion Laminin Rabbits Rate of corneal repair Xyloglucan Integrin 03 medical and health sciences chemistry.chemical_compound Polysaccharides Cornea Burns Chemical medicine Animals Humans Glucans Cells Cultured Corneal epithelium Wound Healing Plants Medicinal 030102 biochemistry & molecular biology biology Epithelium Corneal General Medicine Adhesion Molecular biology eye diseases Ophthalmology Eye Burns 030104 developmental biology medicine.anatomical_structure chemistry Immunology Seeds biology.protein Xylans Ophthalmic Solutions Wound healing Conjunctiva Heptanol |
| Zdroj: | Scopus-Elsevier |
| Popis: | Purpose To explore the role of a natural polysaccharide extracted from tamarind seed (xyloglucan, or tamarind seed polysaccharide, TSP) on the integrin-substrate recognition system and on repair of corneal wounds. Methods a) Cultured human conjunctival cells were labeled by addition of a tritiated amino acid mixture. Their adhesion to laminin-coated culture wells in the absence or presence of TSP was checked by radioactivity count. b) The corneal epithelium of albino rabbits was damaged by applying a paper disc soaked with n-heptanol. The eyes were then treated with TSP, with a hyaluronate reference formulation and with normal saline solution (controls). The diameter of corneal wounds was measured daily, after fluorescein staining. Results Compared to hyaluronate, TSP slightly but significantly increased the wound healing rate. TSP 1.0% exerted a positive influence on cell adhesion to laminin, up to a certain laminin concentration. Conclusions The ability of the polysaccharide to promote corneal wound healing might depend on its influence on the integrin recognition system. |
| Databáze: | OpenAIRE |
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