In vivo behavior of NTBI revealed by automated quantification system
| Autor: | Yasumichi Toki, Addo Lynda, Satoshi Ito, Yutaka Kohgo, Mayumi Hatayama, Daisuke Kato, Naomi Iizuka, Mikihiro Fujiya, Masayo Yamamoto, Kotoe Shibusa, Motohiro Shindo, Katsuya Ikuta, Noriyasu Niizeki |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Quantification methods Iron Physiology 030204 cardiovascular system & hematology Biology Automated quantification system 03 medical and health sciences Sex Factors 0302 clinical medicine In vivo Healthy volunteers Animals Humans Iron overload Serum ferritin Automation Laboratory Inflammation Significant difference Hematology Limiting Organ damage Clinical Practice Non-transferrin bound iron (NTBI) Kinetics 030220 oncology & carcinogenesis Immunology Female Biomarkers |
| Zdroj: | International journal of hematology. 104(2):175-181 |
| ISSN: | 0925-5710 |
| Popis: | The final publication is available at Springer via http://dx.doi.org/10.1007/s12185-016-2002-6. Non-Tf-bound iron (NTBI), which appears in serum in iron overload, is thought to contribute to organ damage; the monitoring of serum NTBI levels may therefore be clinically useful in iron-overloaded patients. However, NTBI quantification methods remain complex, limiting their use in clinical practice. To overcome the technical difficulties often encountered, we recently developed a novel automated NTBI quantification system capable of measuring large numbers of samples. In the present study, we investigated the in vivo behavior of NTBI in human and animal serum using this newly established automated system. Average NTBI in healthy volunteers was 0.44 ± 0.076 μM (median 0.45 μM, range 0.28-0.66 μM), with no significant difference between sexes. Additionally, serum NTBI rapidly increased after iron loading, followed by a sudden disappearance. NTBI levels also decreased in inflammation. The results indicate that NTBI is a unique marker of iron metabolism, unlike other markers of iron metabolism, such as serum ferritin. Our new automated NTBI quantification method may help to reveal the clinical significance of NTBI and contribute to our understanding of iron overload. |
| Databáze: | OpenAIRE |
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