Tissue interactions and estrogenic response during human female fetal reproductive tract development

Autor: Gerald R. Cunha, Stanley J. Robboy, Takeshi Kurita, Paul S. Cooke, Mei Cao, Laurence S. Baskin, Joel Shen
Rok vydání: 2018
Předmět:
0301 basic medicine
Cancer Research
Human female fetal reproductive tract
Uterus
Estrogen receptor
Reproductive health and childbirth
Epithelium
Progesterone receptor
Mesoderm
Mice
0302 clinical medicine
Receptors
2.1 Biological and endogenous factors
Mesenchymal-epithelial interactions
Aetiology
Progesterone
030219 obstetrics & reproductive medicine
Cell Differentiation
Genitalia
Female
medicine.anatomical_structure
Female
Receptors
Progesterone
1.1 Normal biological development and functioning
Mesenchyme
Biology
Article
Paediatrics and Reproductive Medicine
Estrogenic response
Andrology
03 medical and health sciences
Underpinning research
medicine
Animals
Humans
Genitalia
Diethylstilbestrol
Molecular Biology
Fetus
Contraception/Reproduction
Mesenchymal stem cell
Estrogen Receptor alpha
Epithelial Cells
Cell Biology
Estrogen
body regions
030104 developmental biology
Biochemistry and Cell Biology
Estrogen receptor alpha
Developmental Biology
Zdroj: Cunha, GR; Kurita, T; Cao, M; Shen, J; Cooke, PS; Robboy, SJ; et al.(2018). Tissue interactions and estrogenic response during human female fetal reproductive tract development. DIFFERENTIATION, 101, 39-45. doi: 10.1016/j.diff.2018.04.002. UCSF: Retrieved from: http://www.escholarship.org/uc/item/6xf8x2cx
ISSN: 0301-4681
DOI: 10.1016/j.diff.2018.04.002
Popis: The role of tissue interactions was explored to determine whether epithelial differentiation within the developing human reproductive tract is induced and specified by mesenchyme in tissue recombinants composed of mouse vaginal mesenchyme + human uterine tubal epithelium (mVgM+hTubE). The tissue recombinants were grown in DES-treated ovariectomized athymic mice. After 2 to 4 weeks of in vivo growth, several vaginal specific features were expressed in the human tubal epithelium. The mesenchyme-induced effects included morphological change as well as expression of several immunohistochemical markers. Although the mesenchyme-induced shift in vaginal differentiation in the human tubal epithelium was not complete, the partial induction of vaginal markers in human tubal epithelium verifies the importance of mesenchymal-epithelial interactions in development of the human female reproductive tract. In a separate experiment, DES-induction of uterine epithelial progesterone receptor (PGR) and estrogen receptor 1 (ESR1) was explored in tissue recombinants composed of wild-type or Esr1KO mouse uterine mesenchyme + human fetal uterine epithelium (wt UtM+hUtE and Esr1KO UtM+hUtE). The rationale of this experiment was to determine whether DES-induction of PGR and ESR1 is mediated directly via epithelial ESR1 or indirectly (paracrine mechanism) via mesenchymal ESR1. DES-induction of uterine epithelial ESR1 and PGR in Esr1KO UtM+hUtE tissue recombinants (devoid of mesenchymal ESR1) formally eliminates the paracrine mechanism and demonstrates that DES induction of human uterine epithelial ESR1 and PGR is directly mediated via epithelial ESR1.
Databáze: OpenAIRE
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