Superior neovascularization and muscle regeneration in ischemic skeletal muscles following VEGF gene transfer by rAAV1 pseudotyped vectors
| Autor: | Peng Zhang, Wei Gao, Hua Yan, Jianwei Tian, Xiaobing Wu, Xiaolong Fan, Ling-yun Zu, Yanhong Guo, Li Chen, Nanping Wang, Xiao-yan Dong |
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| Rok vydání: | 2005 |
| Předmět: |
Vascular Endothelial Growth Factor A
Genetic enhancement Biophysics Ischemia Neovascularization Physiologic Gene delivery Biology medicine.disease_cause Biochemistry Adenoviridae Cell Line Neovascularization chemistry.chemical_compound Mice Gene expression medicine Animals Humans Regeneration Muscle Skeletal Molecular Biology Adeno-associated virus DNA Primers Recombination Genetic Base Sequence Reverse Transcriptase Polymerase Chain Reaction Regeneration (biology) Gene Transfer Techniques Cell Biology medicine.disease Molecular biology Cell biology Vascular endothelial growth factor chemistry medicine.symptom |
| Zdroj: | Biochemical and biophysical research communications. 336(1) |
| ISSN: | 0006-291X |
| Popis: | Recombinant adeno-associated virus serotype 2 (rAAV2) vector has been widely employed for gene therapy. Recent progress suggests that the new serotypes of AAV showed a better performance than did AAV2 in normal tissues. Here, we evaluate the potential role of human vascular endothelial growth factor (VEGF) gene transfer using rAAV vector pseudotyped with serotype 1 capsid proteins (rAAV1) in the treatment of muscle ischemia. In ischemic skeletal muscles, the rAAV1-LacZ vector allowed higher level, broader distribution, and long-lasting gene expression compared with the rAAV2-LacZ vector. Muscle VEGF165 production following the rAAV1-VEGF165 vector injection was 5–10 times higher than that following the rAAV2-VEGF165 vector injection. VEGF165 production mediated by the rAAV1-VEGF165 vector stimulated a large set of neovascularization with relatively mature vascular structures and enhanced muscle regeneration in the ischemic skeletal muscles. Thus, the rAAV1-VEGF165 vector mediated gene transfer may be a therapeutic approach to peripheral vascular diseases. |
| Databáze: | OpenAIRE |
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