Biological responses to multilayered DNA-coatings
| Autor: | Rjm Roeland Nolte, Mrj Matthijn Vos, XF Walboomers, Najm Nico Sommerdijk, J.A. Jansen, van den Jjjp Beucken |
|---|---|
| Přispěvatelé: | Chemical Engineering and Chemistry, Macromolecular and Organic Chemistry |
| Rok vydání: | 2007 |
| Předmět: |
Male
Chemical and physical biology [NCMLS 7] Tissue engineering and reconstructive surgery [UMCN 4.3] Materials science Biomedical Engineering Inflammation Biomaterials Dermal fibroblast Coated Materials Biocompatible Implants Experimental In vivo Materials Testing medicine Polyamines Animals Polylysine Rats Wistar Cell Proliferation Skin Soft tissue Capsule Biological activity DNA Prostheses and Implants Fibroblasts Tissue engineering and pathology [NCMLS 3] Immunohistochemistry In vitro Actins Rats Histocompatibility Microscopy Electron Scanning Implant Glass medicine.symptom Physical Organic Chemistry Biomedical engineering Immunity infection and tissue repair [NCMLS 1] |
| Zdroj: | Journal of Biomedical Materials Research Part B-Applied Biomaterials, 81, 1, pp. 231-8 Journal of Biomedical Materials Research, 81B, 1, pp. 231-238 Journal of Biomedical Materials Research Part B-Applied Biomaterials, 81, 231-8 Journal of Biomedical Materials Research, 81B, 231-238 Journal of Biomedical Materials Research, Part B: Applied Biomaterials, 81B(1), 231-238. Wiley |
| ISSN: | 1552-4973 0021-9304 |
| Popis: | Contains fulltext : 35076.pdf (Publisher’s version ) (Closed access) This study was performed to evaluate the basic biological response to deoxyribonucleic acid (DNA)-based coatings for soft tissue implants. To that end, in vitro experiments were used to study their cytocompatibility, and in vivo subcutaneous implantation studies with transponders in a rat model were performed to evaluate their histocompatibility. The DNA-based coatings were fabricated using the electrostatic self-assembly technique using cationic poly-D-lysine or poly-allylamine hydrochloride and anionic DNA. Noncoated substrates served as controls. In vitro, the behavior of primary rat dermal fibroblasts was assessed in terms of cell proliferation and morphology. Both types of multilayered DNA-coatings significantly increased rat dermal fibroblast proliferation without altering the morphological appearance of the cells. The tissue response to multilayered DNA-coatings was assessed using an in vivo rat model, in which transponders were inserted subcutaneously for 4 and 12 weeks. No macroscopic signs of inflammation or adverse tissue reactions were observed at implant retrieval. Histological analyses demonstrated a uniform tissue response to all types of implants. All implants were encapsulated in a fibrous tissue capsule without intervening inflammatory cells at the implant surface. Histomorphometrically, multilayered DNA-coatings induced fibrous tissue capsules with similar quality and thickness compared to noncoated controls. In addition, all fibrous tissue capsules showed similar expression of alpha-smooth muscle actin. This study demonstrates that multilayered DNA-coatings are cytocompatible and histocompatible, and justifies further research on their functionalization with biologically active compounds to modulate tissue responses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text