Evolutionary Expansion of WRKY Gene Family in Banana and Its Expression Profile during the Infection of Root Lesion Nematode, Pratylenchus coffeae
| Autor: | Sriram Viswanathan, Saraswathi Marimuthu Somasundram, Mayilvaganan Muthu, Raja Kaliyappan, Backiyarani Suthanthiram, Uma Subbaraya |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Nonsynonymous substitution Nematoda lcsh:Medicine Gene Expression Bananas Plant Science Plant Genetics 01 natural sciences Biochemistry Plant Roots Gene Expression Regulation Plant Gene duplication Medicine and Health Sciences Plant Genomics lcsh:Science Nematode Infections Disease Resistance Plant Proteins Genetics Multidisciplinary biology Chromosome Biology food and beverages Agriculture Genomics Plants Plant Physiology Multigene Family Sequence Analysis Research Article Biotechnology Crops Plant disease resistance Research and Analysis Methods Chromosomes Fruits 03 medical and health sciences Sequence Motif Analysis DNA-binding proteins Parasitic Diseases Gene family Animals Pratylenchus coffeae Gene Regulation Plant Defenses Molecular Biology Techniques Sequencing Techniques Gene Molecular Biology Plant Diseases lcsh:R Organisms Biology and Life Sciences Proteins Musa Cell Biology Biotic stress biology.organism_classification WRKY protein domain Regulatory Proteins 030104 developmental biology lcsh:Q Plant Biotechnology 010606 plant biology & botany Crop Science Transcription Factors |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 9, p e0162013 (2016) |
| ISSN: | 1932-6203 |
| Popis: | The WRKY family of transcription factors orchestrate the reprogrammed expression of the complex network of defense genes at various biotic and abiotic stresses. Within the last 96 million years, three rounds of Musa polyploidization events had occurred from selective pressure causing duplication of MusaWRKYs with new activities. Here, we identified a total of 153 WRKY transcription factors available from the DH Pahang genome. Based on their phylogenetic relationship, the MusaWRKYs available with complete gene sequence were classified into the seven common WRKY sub-groups. Synteny analyses data revealed paralogous relationships, with 17 MusaWRKY gene pairs originating from the duplication events that had occurred within the Musa lineage. We also found 15 other MusaWRKY gene pairs originating from much older duplication events that had occurred along Arecales and Poales lineage of commelinids. Based on the synonymous and nonsynonymous substitution rates, the fate of duplicated MusaWRKY genes was predicted to have undergone sub-functionalization in which the duplicated gene copies retain a subset of the ancestral gene function. Also, to understand the regulatory roles of MusaWRKY during a biotic stress, Illumina sequencing was performed on resistant and susceptible cultivars during the infection of root lesion nematode, Pratylenchus coffeae. The differential WRKY gene expression analysis in nematode resistant and susceptible cultivars during challenged and unchallenged conditions had distinguished: 1) MusaWRKYs participating in general banana defense mechanism against P.coffeae common to both susceptible and resistant cultivars, 2) MusaWRKYs that may aid in the pathogen survival as suppressors of plant triggered immunity, 3) MusaWRKYs that may aid in the host defense as activators of plant triggered immunity and 4) cultivar specific MusaWRKY regulation. Mainly, MusaWRKY52, -69 and -92 are found to be P.coffeae specific and can act as activators or repressors in a defense pathway. Overall, this preliminary study in Musa provides the basis for understanding the evolution and regulatory mechanism of MusaWRKY during nematode stress. |
| Databáze: | OpenAIRE |
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