Prejunctional neuropeptide Y receptors in human kidney and atrium
| Autor: | M C Michel, E Schwertfeger, M Riess, L C Rump, P Schollmeyer, C Bohmann |
|---|---|
| Přispěvatelé: | Other departments |
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Kidney Cortex Renal cortex Biology In Vitro Techniques Pertussis toxin Kidney chemistry.chemical_compound Norepinephrine GTP-Binding Proteins Internal medicine mental disorders medicine Animals Humans Neuropeptide Y Heart Atria Virulence Factors Bordetella Receptor Neurotransmitter Pharmacology Myocardium Kidney metabolism Neuropeptide Y receptor Atrial Function humanities Electric Stimulation Receptors Neuropeptide Y medicine.anatomical_structure Endocrinology chemistry Pertussis Toxin Renal physiology Peptide YY Rabbits Cardiology and Cardiovascular Medicine |
| Zdroj: | Journal of cardiovascular pharmacology, 29(5), 656-661. Lippincott Williams and Wilkins |
| ISSN: | 0160-2446 |
| Popis: | The aim of our study was to characterize functionally prejunctional neuropeptide Y (NPY) receptors in human and rabbit renal cortex, as well as in human right atrium. Segments of human atrial appendages and of human and rabbit renal cortex were preincubated with [3H]noradrenaline, superfused with Krebs-Henseleit solution and stimulated electrically in superfusion chambers. The stimulation-induced outflow of radioactivity was taken as an index of endogenous noradrenaline release. The effects of subtype-selective NPY analogs on the stimulation-induced noradrenaline release were studied. NPY, its endogenous analog, peptide YY, and its C-terminal fragment, NPY13-36, but not its analog, [Leu31,Pro34]NPY, concentration dependently (1-100 nM) inhibited [3H]noradrenaline release in all tissues studied. NPY-induced inhibition of [3H]noradrenaline release in human and rabbit kidney was abolished by pretreatment with pertussis toxin. We conclude that prejunctional inhibition of noradrenaline release in human heart and human and rabbit kidney occurs through NPY receptors of the Y2 subtype, which appear to couple to a pertussis toxin-sensitive G protein. |
| Databáze: | OpenAIRE |
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