Variable requirement for splicing signals for nucleocytoplasmic export of mRNAs
| Autor: | Ahmed Waseem, Kenneth N. White, Christine K. M. Suen, Litao Fu |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cytoplasm
DNA Complementary RNA Splicing Genetic Vectors Clinical Biochemistry Exonic splicing enhancer Biology Biochemistry Cricetinae Receptors Adrenergic beta Genetics medicine Animals Humans RNA Messenger Molecular Biology Gene In Situ Hybridization Cell Nucleus Messenger RNA Transferrin Intron Ceruloplasmin Cell Biology Molecular biology Introns Recombinant Proteins Neoplasm Proteins Cell biology Cell nucleus medicine.anatomical_structure Receptors Serotonin COS Cells RNA splicing Cytokines Keratins Expression cassette Receptors Serotonin 5-HT1 |
| Zdroj: | IUBMB Life. 42:329-337 |
| ISSN: | 1521-6543 |
| DOI: | 10.1080/15216549700202731 |
| Popis: | Using in situ hybridisation to detect the intracellular localisation of mRNAs we have found that mRNAs expressed from intronless cDNAs of normally intronic genes are expressed well but largely retained in nuclei. The degree of nuclear retention is quite variable but in all cases addition of splicing signals to the expression cassette are required for efficient export of the mRNAs from nucleus to cytoplasm. In contrast mRNAs expressed from the intronless genes of hamster beta-adrenergic receptor and human serotonin receptor type 1A showed very little nuclear accumulation and strong expression in the cytoplasm independently of splicing signals. The data demonstrate a link between splicing and export and dissemble from the idea that splicing enhances mRNA expression by protecting nascent nuclear mRNAs from degradation. |
| Databáze: | OpenAIRE |
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