Ocular toxicity study of trypan blue injected into the vitreous cavity of rabbit eyes
| Autor: | H. Spekreijse, Jan Monzer, Karin Kobuch, Jan C. van Meurs, Marc Veckeneer, Wilfried Van Marle, Koen A van Overdam |
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| Rok vydání: | 2001 |
| Předmět: |
Rhodopsin
medicine.medical_specialty genetic structures medicine.medical_treatment Lens Capsule Crystalline Vitrectomy Cataract Retina Ophthalmoscopy Cellular and Molecular Neuroscience chemistry.chemical_compound Ophthalmology Electroretinography medicine Animals Coloring Agents medicine.diagnostic_test business.industry Retinal Degeneration Histology Trypan Blue Anatomy medicine.disease Immunohistochemistry eye diseases Sensory Systems Staining Vitreous Body Ki-67 Antigen chemistry Trypan blue Rabbits sense organs Ophthalmic Solutions Epiretinal membrane business Retinopathy |
| Zdroj: | Graefe's Archive for Clinical and Experimental Ophthalmology. 239:698-704 |
| ISSN: | 0721-832X |
| DOI: | 10.1007/s004170100341 |
| Popis: | Background: To evaluate the ocular toxicity of trypan blue (TB) injected into the vitreous cavity of rabbit eyes. TB is a dye that could be useful for staining epiretinal membranes during vitrectomy surgery. Methods: Ten New Zealand White (NZW) rabbits underwent gas-compression vitrectomy. Rabbits were divided into three groups to receive injections of 0.1 ml basic salt solution, 0.1 ml of a 0.06% TB solution or 0.1 ml of a 0.2% TB solution. Ocular toxicity was assessed by slit-lamp biomicroscopy, ophthalmoscopy, electroretinography and histology. Results: Transient posterior capsule opacification was noted in all animals. No significant reductions in a-wave or b-wave amplitudes were found in any of the animals. Light and electron microscopic examination of the inferior retina in the 0.2% TB-treated eyes showed damaged photoreceptors and marked disorganization. Immunohistochemical staining for rhodopsin was strongly reduced in those sections and staining for proliferation with Ki-67 was positive. No histological abnormalities were found in the upper retina of the 0.2% TB-treated eyes or in any part of the retina of the 0.06% TB-treated or control eyes. No histological abnormalities were found in any of the anterior chamber angle specimens. Conclusions: Although no signs of toxicity were found after the prolonged presence of TB at a concentration of 0.06% in the vitreous cavity of rabbit eyes, marked damage occurred in the lower retina of 0.2% TB-treated eyes. The short-term presence of TB at a concentration of 0.06% in the vitreous cavity is harmless to the rabbit eye but a higher concentration of TB could be unsafe. |
| Databáze: | OpenAIRE |
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