Co-delivery of genes can be confounded by bicistronic vector design

Autor: Hanieh Moradian, Manfred Gossen, Andreas Lendlein
Rok vydání: 2022
Předmět:
Zdroj: Moradian, H.; Gossen, M.; Lendlein, A.: Co-delivery of genes can be confounded by bicistronic vector design. In: MRS Communications. Vol. 12 (2022) 145-153. (DOI: /10.1557/s43579-021-00128-7)
ISSN: 2159-6867
2159-6859
Popis: Maximizing the efficiency of nanocarrier-mediated co-delivery of genes for co-expression in the same cell is critical for many applications. Strategies to maximize co-delivery of nucleic acids (NA) focused largely on carrier systems, with little attention towards payload composition itself. Here, we investigated the effects of different payload designs: co-delivery of two individual “monocistronic” NAs versus a single bicistronic NA comprising two genes separated by a 2A self-cleavage site. Unexpectedly, co-delivery via the monocistronic design resulted in a higher percentage of co-expressing cells, while predictive co-expression via the bicistronic design remained elusive. Our results will aid the application-dependent selection of the optimal methodology for co-delivery of genes. Graphical abstract
Databáze: OpenAIRE
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