A New Player in Jasmonate-Mediated Stomatal Closure: The Arabidopsis thaliana Copper Amine Oxidase β

Autor: Alessandra Cona, Paraskevi Tavladoraki, Riccardo Angelini, Ilaria Fraudentali, Chiara Pedalino
Přispěvatelé: Fraudentali, I., Pedalino, C., Tavladoraki, P., Angelini, R., Cona, A.
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Polyamine
Mutant
Oxylipin
Arabidopsis
Methyl jasmonate
chemistry.chemical_compound
Stomatal closure
Plant Growth Regulators
Gene Expression Regulation
Plant
Guard cell
Arabidopsis thaliana
Jasmonate
Ethylamine
Biology (General)
biology
Chemistry
Plant Growth Regulator
Thiourea
General Medicine
copper amine oxidases
stomatal closure
Apoplast
Cell biology
Copper amine oxidase
Amine Oxidase (Copper-Containing)
Reactive Oxygen Specie
polyamines
hydrogen peroxide
hormones
methyl jasmonate
stress response
Signal Transduction
QH301-705.5
chemistry.chemical_element
Arabidopsis Protein
Cyclopentanes
Calcium
Nitric Oxide
Article
Nitric oxide
Ethylamines
Oxylipins
Cyclopentane
Arabidopsis Proteins
Stress response
fungi
Hydrogen Peroxide
biology.organism_classification
Hormone
Plant Stomata
Arabidopsi
Reactive Oxygen Species
Abscisic Acid
Zdroj: Cells
Cells, Vol 10, Iss 3399, p 3399 (2021)
Cells; Volume 10; Issue 12; Pages: 3399
ISSN: 2073-4409
Popis: Plant defence responses to adverse environmental conditions include different stress signalling, allowing plant acclimation and survival. Among these responses one of the most common, immediate, and effective is the modulation of the stomatal aperture, which integrates different transduction pathways involving hydrogen peroxide (H2O2), calcium (Ca2+), nitric oxide (NO), phytohormones and other signalling components. The Arabidopsis thaliana copper amine oxidases β (AtCuAOβ) encodes an apoplastic CuAO expressed in guard cells and root protoxylem tissues which oxidizes polyamines to aminoaldehydes with the production of H2O2 and ammonia. Here, its role in stomatal closure, signalled by the wound-associated phytohormone methyl-jasmonate (MeJA) was explored by pharmacological and genetic approaches. Obtained data show that AtCuAOβ tissue-specific expression is induced by MeJA, especially in stomata guard cells. Interestingly, two Atcuaoβ T-DNA insertional mutants are unresponsive to this hormone, showing a compromised MeJA-mediated stomatal closure compared to the wild-type (WT) plants. Coherently, Atcuaoβ mutants also show compromised H2O2-production in guard cells upon MeJA treatment. Furthermore, the H2O2 scavenger N,N1-dimethylthiourea (DMTU) and the CuAO-specific inhibitor 2-bromoethylamine (2-BrEtA) both reversed the MeJA-induced stomatal closure and the H2O2 production in WT plants. Our data suggest that AtCuAOβ is involved in the H2O2 production implicated in MeJA-induced stomatal closure.
Databáze: OpenAIRE
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