Suppressor of cytokine signalling protein SOCS3 expression is increased at sites of acute and chronic inflammation

Autor: David R. Greaves, Mark R. Addley, Andrew Cotterill, Elizabeth J. Soilleux, Gemma E. White
Přispěvatelé: Soilleux, Elizabeth [0000-0002-4032-7249], Apollo - University of Cambridge Repository
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Male
Macrophage
Physiology
Gene Expression
Expression
Suppressor of Cytokine Signaling Proteins
0302 clinical medicine
Genes
Reporter
Cricetinae
SOCS3
Luciferases
0303 health sciences
Granuloma
digestive
oral
and skin physiology
Acute-phase protein
JAK-STAT signaling pathway
General Medicine
Up-Regulation
3. Good health
Protein Transport
Organ Specificity
Female
medicine.symptom
Histology
Sarcoidosis
Giant Cell Arteritis
Inflammation
CHO Cells
Biology
Suppressor of cytokine signalling
03 medical and health sciences
Cricetulus
medicine
Animals
Humans
Acute-Phase Reaction
030304 developmental biology
Original Paper
Tissue
HEK 293 cells
Cell Biology
Appendicitis
Inflammatory Bowel Diseases
beta-Galactosidase
Molecular biology
HEK293 Cells
Suppressor of Cytokine Signaling 3 Protein
Transcription factor
Biomarkers
Immunostaining
030215 immunology
Zdroj: Journal of Molecular Histology
ISSN: 1567-2387
1567-2379
Popis: Treatment of cells with cytokines and growth factors leads to the synthesis of Suppressor of Cytokine Signalling (SOCS) proteins that act as potent negative regulators of signalling via the Jak/STAT pathway. We used immunohistochemistry to identify cells and pathologies where SOCS3 expression might influence acute and chronic inflammatory responses in human tissues. Epitope and GFP tagged SOCS3 fusion proteins were localised predominantly in the nucleus of transfected cells and a validated anti SOCS3 antiserum revealed the expression of SOCS3 in the nucleus and cytoplasm of macrophages, endothelial and epithelial cells in a wide range of normal tissues in tissue microarrays (n = 31 different tissues). Nuclear SOCS3 was only seen in cells expressing a high level of the protein. Comparative immunostaining of acute, chronically and granulomatously inflamed human tissues revealed higher levels of nuclear and cytoplasmic SOCS3 expression in inflamed than in corresponding normal tissues, particularly in recruited leukocyte populations, but also in epithelia. The staining appeared more intense, suggesting higher expression levels, in areas where inflammation was more acute, consistent with the time course of SOCS3 induction described in vitro. Expression of SOCS3 protein by leucocytes and other cell types in tissue sections could be a useful marker of cells undergoing acute or chronic stimulation by cytokines in vivo.
Databáze: OpenAIRE
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