Obesity impairs resistance to Leishmania major infection in C57BL/6 mice
| Autor: | Leda Quercia Vieira, Leonardo Gomes Vaz, Lícia Torres, Nivea Carolina Paiva, Franciele Carolina Silva, Tatiani Uceli Maioli, Graziele Ribeiro Goes, Cláudia Martins Carneiro, Felipe Caixeta, Sara Cândida Barbosa, Ana Maria Caetano Faria, Matheus B. H. Carneiro, Vinicius Dantas Martins |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Physiology RC955-962 Otology Ear Infections Pathology and Laboratory Medicine White Blood Cells 0302 clinical medicine Risk Factors Animal Cells Immune Physiology Arctic medicine. Tropical medicine Medicine and Health Sciences Leishmania major Immune Response Lymph node Mice Knockout Innate Immune System biology Interleukin-17 Ear Infectious Diseases medicine.anatomical_structure Physiological Parameters Cytokines Female Cellular Types Anatomy medicine.symptom Public aspects of medicine RA1-1270 Research Article Immune Cells Immunology 030231 tropical medicine Ear infection Leishmaniasis Cutaneous Context (language use) Inflammation Lymphatic System Lesion Interferon-gamma 03 medical and health sciences Signs and Symptoms Immune system Diagnostic Medicine Immunity Parasitic Diseases medicine Animals Obesity Nutrition Blood Cells business.industry Macrophages Body Weight Public Health Environmental and Occupational Health Biology and Life Sciences Cell Biology Molecular Development biology.organism_classification Diet Mice Inbred C57BL 030104 developmental biology Otorhinolaryngology Immune System Macrophages Peritoneal Lymph Nodes business Developmental Biology |
| Zdroj: | Repositório Institucional da UFOP Universidade Federal de Ouro Preto (UFOP) instacron:UFOP PLoS Neglected Tropical Diseases, Vol 14, Iss 1, p e0006596 (2020) PLoS Neglected Tropical Diseases |
| Popis: | An association between increased susceptibility to infectious diseases and obesity has been described as a result of impaired immunity in obese individuals. It is not clear whether a similar linkage can be drawn between obesity and parasitic diseases. To evaluate the effect of obesity in the immune response to cutaneous Leishmania major infection, we studied the ability of C57BL/6 mice fed a hypercaloric diet (HSB) to control leishmaniasis. Mice with diet-induced obesity presented thicker lesions with higher parasite burden and a more intense inflammatory infiltrate in the infected ear after infection with L. major. There was no difference between control and obese mice in IFN-gamma or IL-4 production by auricular draining lymph node cells, but obese mice produced higher levels of IgG1 and IL-17. Peritoneal macrophages from obese mice were less efficient to kill L. major when infected in vitro than macrophages from control mice. In vitro stimulation of macrophages with IL-17 decreased their capacity to kill the parasite. Moreover, macrophages from obese mice presented higher arginase activity. To confirm the role of IL-17 in the context of obesity and infection, we studied lesion development in obese IL-17R-/- mice infected with L. major and found no difference in skin lesions and the leukocyte accumulation in the draining lymph node is redcuced in knockout mice compared between obese and lean animals. Our results indicate that diet-induced obesity impairs resistance to L. major in C57BL/6 mice and that IL-17 is involved in lesion development. Author summary Obesity is a serious and increasing public health problem, and also induces a spectrum of metabolic disorders. Some diseases are known to be more severe in the presence of obesity. However, the interactions of obesity with the immune response to infectious agents have not been fully explored. In this study, we investigated the response of obese mice to infection with Leishmania major. C57BL/6 mice were fed a hypercaloric diet (HSB) and infected afterward with L. major. In obese mice, lesions were ticker and more ulcerative, and cells from draining lymph nodes produced more IL-17 when compared with cells from lean mice fed a control diet. Macrophages from obese and lean mice were infected in vitro and stimulated with IL-17 to test the role of this cytokine in effect produced by obesity. Macrophages from obese mice were more infected by L. major than the macrophages from control mice and the number of parasites was increased by treatment with IL-17. IL-17R deficient mice treated with hypercaloric diet showed no difference in lesion size when compared to mice fed control diet. Our findings suggest that diet-induced obesity decrease the resistance to L. major infection of C57BL/6 mice and the IL-17 cytokine may be involved in the lesion formation. |
| Databáze: | OpenAIRE |
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