Regeneration and tolerance factor is expressed during T-lymphocyte activation and plays a role in apoptosis
| Autor: | Kenneth D. Beaman, Richard Derks, Brian K. DuChateau, Gerald W. Lee, Alice Gilman-Sachs, Jonathan S. Boomer |
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| Rok vydání: | 2001 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte T-Lymphocytes Receptor expression Immunology Apoptosis Pregnancy Proteins Lymphocyte Activation Jurkat cells Fas ligand Jurkat Cells Antigens CD Suppressor Factors Immunologic Humans Immunology and Allergy Lectins C-Type fas Receptor IL-2 receptor Annexin A5 CD40 biology Cell Membrane T-cell receptor Antibodies Monoclonal Receptors Interleukin-2 General Medicine T lymphocyte Fas receptor Molecular biology Kinetics Leukocytes Mononuclear biology.protein |
| Zdroj: | Human Immunology. 62:577-588 |
| ISSN: | 0198-8859 |
| Popis: | Regeneration and tolerance factor (RTF) is a protein cloned from the thymus and expressed on B lymphocytes in normal pregnancy, B lymphocytic leukemia lines, and T and B lymphocytes in individuals with HIV infection. Findings, using the Jurkat T-cell model, revealed that RTF is upregulated after activation and anti-RTF antibody-induced apoptosis. In this article anti-RTF antibody-induced apoptosis of both unstimulated and activated T lymphocytes. RTF expression was examined in human PBMC or purified T lymphocytes after their in vitro activation. Kinetic studies indicated maximal RTF cell surface expression on activated T lymphocytes occurred between expression of the early activation antigen CD69 and the IL-2α receptor (CD25) by multiparameter flow cytometry. RTF receptor expression correlated with Fas (CD95) and CD25 receptor expression (r 2 = 0.6 and 0.5, respectively). RTF surface expression was dependent on the stimuli used to activate T lymphocytes. T lymphocytes obtained maximal RTF expression when activated through the TCR signal complex using anti-CD3e antibody alone when compared with T lymphocytes activated with costimulation provided by anti-CD28 antibody alone or with anti-CD28 and anti-CD3e antibody. RTF is expressed under conditions of both activation and anergy. The RTFs increased concentration on the surface of anergic T cells may protect these cells from apoptosis because increased RTF concentrations inhibited anti-RTF induced apoptosis. These data further characterize the expression of RTF on activated T lymphocytes and the role of anti-RTF antibody in T-lymphocyte apoptosis. |
| Databáze: | OpenAIRE |
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