Involvement of contact sites in phosphatidylserine import into liver mitochondria
| Autor: | Dominique Ardail, F. Lerme, Pierre Louisot |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Decarboxylation
Mitochondria Liver Phosphatidylserines Biology Mitochondrion Biochemistry chemistry.chemical_compound Mice Inner membrane Animals Freeze Fracturing Inner mitochondrial membrane Molecular Biology Phosphatidylethanolamine Binding Sites Phosphatidylethanolamines Biological Transport Cell Biology Phosphatidylserine Intracellular Membranes Membrane transport Cell Compartmentation Kinetics chemistry Biophysics Phosphatidylserine decarboxylase |
| Zdroj: | The Journal of biological chemistry. 266(13) |
| ISSN: | 0021-9258 |
| Popis: | The synthesis, translocation, and decarboxylation of phosphatidylserine can occur in a cell-free system (Voelker, D. R. (1989) J. Biol. Chem. 264, 8019-8025). We made use of the spatial separation of the site of biosynthesis and the site of decarboxylation of phosphatidylserine to demonstrate that mitochondrial contact sites are intimately involved in the translocation of phosphatidylserine prior to decarboxylation. In that sense, the inhibition of phosphatidylserine decarboxylase leads to an accumulation of this phospholipid in the contact site-enriched fractions without mixing the inner membrane phospholipid pool. On the other hand, newly synthesized phosphatidylethanolamine can be exported very rapidly to the mitochondrial surface in the same way, i.e. via contact sites. These data provide further evidence for the existence of a structural and functional microcompartmentation at the inner mitochondrial membrane surface. |
| Databáze: | OpenAIRE |
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