Polyelectrolyte-Coated Gold Magnetic Nanoparticles for Immunoassay Development: Toward Point of Care Diagnostics for Syphilis Screening
| Autor: | Yang Dong, Yali Cui, Ma Jianzhong, Jiangcun Yang, Yanling Luo, Paul Ananda Raju, Mingli Peng, Wenli Hui, Qinlu Zhang, Chao Chen, Ningning Li |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Immunoassay
Thermogravimetric analysis medicine.diagnostic_test Polymers Point-of-Care Systems Metal Nanoparticles Nanoparticle Nanotechnology Polyelectrolyte Analytical Chemistry chemistry.chemical_compound chemistry Spectrophotometry medicine Mass Screening Particle Magnetic nanoparticles Gold Syphilis Acrylic acid |
| Zdroj: | Analytical Chemistry. 85:6688-6695 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac400517e |
| Popis: | Immediate response for disease control relies on simple, inexpensive, and sensitive diagnostic tests, highly sought after for timely and accurate test of various diseases, including infectious diseases. Composite Fe3O4/Au nanoparticles have attracted considerable interest in diagnostic applications due to their unique physical and chemical properties. Here, we developed a simple coating procedure for gold magnetic nanoparticles (GMNs) with poly(acrylic acid) (PAA). PAA-coated GMNs (PGMNs) were stable and monodispersed and characterized by Fourier transform-infrared spectroscopy (FT-IR), transmission electron microscopy, UV-visible scanning spectrophotometry, thermogravimetric analysis, and Zetasizer methodologies. For diagnostic application, we established a novel lateral flow immunoassay (LFIA) strip test system where recombinant Treponema pallidum antigens (r-Tp) were conjugated with PGMNs to construct a particle probe for detection of anti-Tp antibodies. Intriguingly, the particle probes specifically identified Tp antibodies with a detection limitation as low as 1 national clinical unit/mL (NCU/mL). An ample pool of 1020 sera samples from three independent hospitals were obtained to assess our PGMNs-based LFIA strips, which exhibited substantially high values of sensitivity and specificity for all clinical tests (higher than 97%) and, therefore, proved to be a suitable approach for syphilis screening at a point-of-care test manner. |
| Databáze: | OpenAIRE |
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