Systematic Synthesis of Inhibitors of the Two First Enzymes of the Bacterial Heptose Biosynthetic Pathway: Towards Antivirulence Molecules Targeting Lipopolysaccharide Biosynthesis
| Autor: | Stéphanie Floquet, Maxime Durka, Stéphane P. Vincent, Mounir Andaloussi, Régis Périon, Elodie Malacain, Vincent Gerusz, Michael Bosco, Mayalen Oxoby, Abdellatif Tikad, François Moreau |
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| Rok vydání: | 2011 |
| Předmět: |
Lipopolysaccharides
Antivirulence Molecular Sequence Data Heptose Virulence Isomerase Catalysis Cell wall Bacterial Proteins Escherichia coli Humans Molecule Enzyme Inhibitors Phosphorylation Isomerases chemistry.chemical_classification Molecular Structure Kinase Escherichia coli Proteins Phosphotransferases Organic Chemistry Stereoisomerism General Chemistry Heptoses Biosynthetic Pathways Enzyme chemistry Biochemistry |
| Zdroj: | Chemistry - A European Journal. 17:11305-11313 |
| ISSN: | 0947-6539 |
| DOI: | 10.1002/chem.201100396 |
| Popis: | L-Heptoses (L-glycero-D-manno-heptopyranoses) are constituents of the inner core of lipolysaccharide (LPS), a molecule playing key roles in the mortality of many infectious diseases as well as in the virulence of many human pathogens. The inhibition of the first enzymes of the bacterial heptose biosynthetic pathway is an almost unexplored field to date although it appears to be a very novel way for the development of antivirulence drugs. We report the synthesis of a series of D-glycero-D-manno-heptopyranose 7-phosphate (H7P) analogues and their inhibition properties against the isomerase GmhA and the the kinase HldE, the two first enzymes of the bacterial heptose biosynthetic pathway. The heptose structures have been modified at the 1-, 2-, 6- and 7-positions to probe the importance of the key structural features of H7P that allow a tight binding to the target enzymes; H7P being the product of GmhA and the substrate of HldE, the second objective was to find structures that could simultaneously inhibit both enzymes. We found that GmhA and HldE were extremely sensitive to structural modifications at the 6- and 7- positions of the heptose scaffold. To our surprise, the epimeric analogue of H7P displaying a D-glucopyranose configuration was found to be the best inhibitor of both enzymes but also the only molecule of this series that could inhibit GmhA (IC(50)=34 μM) and HldE (IC(50)=9.4 μM) in the low micromolar range. Noteworthy, this study describes the first inhibitors of GmhA ever reported, and paves the way to the design of a second generation of molecules targeting the bacterial virulence. |
| Databáze: | OpenAIRE |
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