Identification of a mutation causing a defective spindle assembly checkpoint in high ethyl caproate-producing sake yeast strain K1801
| Autor: | Yoshikazu Ohya, Ryo Nakamura, Masaki Mizunuma, Tetsuya Goshima, Hitoshi Shimoi, Dai Hirata, Kazunori Kume, Naoto Okazaki, Masaaki Inahashi, Hiroyasu Tamura, Eri Ichikawa, Takeshi Akao, Hirokazu Hasuda, Hiroki Okada |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Protein subunit Cell Gene Expression Cell Cycle Proteins Saccharomyces cerevisiae Biology Polymorphism Single Nucleotide Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry 03 medical and health sciences Japan medicine Humans Amino Acid Sequence Protein Phosphatase 2 Selection Genetic Caproates Molecular Biology Gene Genetics Base Sequence Ethanol 030102 biochemistry & molecular biology Alcoholic Beverages Organic Chemistry Oryza General Medicine Protein phosphatase 2 Yeast Spindle checkpoint 030104 developmental biology medicine.anatomical_structure Ethyl caproate Fermentation Mutation Odorants Mutation (genetic algorithm) Food Technology M Phase Cell Cycle Checkpoints Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 80:1657-1662 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2016.1184963 |
| Popis: | In high-quality sake brewing, the cerulenin-resistant sake yeast K1801 with high ethyl caproate-producing ability has been used widely; however, K1801 has a defective spindle assembly checkpoint (SAC). To identify the mutation causing this defect, we first searched for sake yeasts with a SAC-defect like K1801 and found that K13 had such a defect. Then, we searched for a common SNP in only K1801 and K13 by examining 15 checkpoint-related genes in 23 sake yeasts, and found 1 mutation, R48P of Cdc55, the PP2A regulatory B subunit that is important for the SAC. Furthermore, we confirmed that the Cdc55-R48P mutation was responsible for the SAC-defect in K1801 by molecular genetic analyses. Morphological analysis indicated that this mutation caused a high cell morphological variation. But this mutation did not affect the excellent brewing properties of K1801. Thus, this mutation is a target for breeding of a new risk-free K1801 with normal checkpoint integrity. |
| Databáze: | OpenAIRE |
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