Reduced CD4 T Lymphocytes in Lymph Nodes of the Mouse Model of Autism Induced by Valproic Acid
| Autor: | Diego Baronio, Wilson Savino, Rudimar dos Santos Riesgo, Daniella Arêas Mendes-da-Cruz, Gustavo Della-Flora Nunes, Carmem Gottfried, Kamila Castro, Guilherme Bauer-Negrini, Victorio Bambini-Junior |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male 0301 basic medicine medicine.medical_specialty medicine.medical_treatment Brachial lymph nodes CD3 T cell Immunology Intraperitoneal injection Spleen Mice 03 medical and health sciences Endocrinology Pregnancy Internal medicine medicine Animals Autistic Disorder Enzyme Inhibitors Valproic Acid biology Endocrine and Autonomic Systems business.industry B230 3. Good health Disease Models Animal 030104 developmental biology Lymphatic system medicine.anatomical_structure Neurology Prenatal Exposure Delayed Effects biology.protein Female Lymph Nodes Lymph business medicine.drug |
| Zdroj: | Neuroimmunomodulation. 25:280-284 |
| ISSN: | 1423-0216 1021-7401 |
| Popis: | Objective: Considering the potential role of lymphocytes in the pathophysiology of autism spectrum disorder (ASD), we aimed to evaluate possible alterations of T cell pools in the lymphoid organs of an animal model of autism induced by valproic acid (VPA). Pregnant Swiss mice received a single intraperitoneal injection of 600 mg/kg of VPA (VPA group) or saline (control group) on day 11 of gestation. Male offspring were euthanized on postnatal day 60 for removal of thymuses, spleens, and a pool of inguinal, axillary and brachial lymph nodes. Cellularity was evaluated, and flow cytometry analysis was performed on cell suspensions incubated with the mouse antibodies anti-CD3-FITC, anti-CD4-PE, and anti-CD8-PE-Cy7. We observed that the prenatal exposure to VPA induced a reduction in the numbers of CD3+CD4+ T cells in their lymph nodes when compared to the control animals. This was specific since it was not seen in the thymus or spleen. The consistent decrease in the number of CD4+ T cells in subcutaneous lymph nodes of mice from the animal model of autism may be related to the allergic symptoms frequently observed in ASD. Further research is necessary to characterize the immunological patterns in ASD and the connection with the pathophysiology of this disorder. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|