Uterotrophic and Hershberger assays for endocrine disruption properties of plastic food contact materials polypropylene (PP) and polyethylene terephthalate (PET)
| Autor: | Seul Min Choi, Duck Soo Lim, Byung-Mu Lee, Bu Young Chung, Hyung Sik Kim, Seong Kwang Lim, Seung Jun Kwack, Minji Kyung |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Health Toxicology and Mutagenesis Food Contamination Endocrine Disruptors Genitalia Male Toxicology Polypropylenes Flutamide Diffusion Rats Sprague-Dawley chemistry.chemical_compound Seminal vesicle Limit of Detection Plasticizers Internal medicine Metals Heavy medicine Polyethylene terephthalate Bioassay Endocrine system Animals Testosterone Chemistry Polyethylene Terephthalates Plasticizer Food Packaging Genitalia Female Organ Size Rats Endocrinology medicine.anatomical_structure Biological Assay Female Orchiectomy Corn oil |
| Zdroj: | Journal of toxicology and environmental health. Part A. 76(10) |
| ISSN: | 1528-7394 |
| Popis: | Plasticizers or plastic materials such as phthalates, bisphenol-A (BPA), and styrene are widely used in the plastic industry and are suspected endocrine-disrupting chemicals (EDC). Although plastic materials such as polypropylene (PP) and polyethylene terephthalate (PET) are not EDC and are considered to be safe, their potential properties as EDC have not been fully investigated. In this study, plastic samples eluted from plastic food containers (PP or PET) were investigated in Sprague-Dawley rats using Hershberger and uterotrophic assays. In the Hershberger assay, 6-wk-old castrated male rats were orally treated for 10 consecutive days with plastic effluent at 3 different doses (5 ml/kg) or vehicle control (corn oil, 1 ml/100 g) to determine the presence of both anti-androgenic and androgenic effects. Testosterone (0.4 mg/ml/kg) was subcutaneously administered for androgenic evaluation as a positive control, whereas testosterone (0.4 mg/ml/kg) and flutamide (3 mg/kg/day) were administered to a positive control group for anti-androgenic evaluation. The presence of any anti-androgenic or androgenic activities of plastic effluent was not detected. Sex accessory tissues such as ventral prostate or seminal vesicle showed no significant differences in weight between treated and control groups. For the uterotrophic assay, immature female rats were treated with plastic effluent at three different doses (5 ml/kg), with vehicle control (corn oil, 1 ml/100 g), or with ethinyl estradiol (3 μg/kg/d) for 3 d. There were no significant differences between test and control groups in vagina or uterine weight. Data suggest that effluents from plastic food containers do not appear to produce significant adverse effects according to Hershberger and uterotrophic assays. |
| Databáze: | OpenAIRE |
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