The effects of di 2-ethyl hexyl phthalate (DEHP) on cellular lipid accumulation in HepG2 cells and its potential mechanisms in the molecular level
Autor: | Wang Zhang, Wei Wei, Hao Chen, Weiping Xu, Xin yue Shen, Wen-wen Zhang |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
endocrine system medicine.medical_specialty Health Toxicology and Mutagenesis Cell Culture Techniques Peroxisome proliferator-activated receptor 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Plasticizers Internal medicine Diethylhexyl Phthalate Malondialdehyde medicine Oil Red O Humans PPAR alpha 0105 earth and related environmental sciences chemistry.chemical_classification Microscopy Confocal biology Dose-Response Relationship Drug Superoxide Dismutase Phthalate Hep G2 Cells Lipid Metabolism Sterol regulatory element-binding protein Oleic acid Oxidative Stress 030104 developmental biology Endocrinology chemistry Biochemistry biology.protein Sterol Regulatory Element Binding Protein 1 Oxidative stress Oleic Acid |
Zdroj: | Toxicology mechanisms and methods. 27(4) |
ISSN: | 1537-6524 |
Popis: | Diethylhexyl phthalate (DEHP) is suspected to be an inevitable factor related to metabolic disease. Our previous study demonstrated that excess DEHP could exacerbate non-alcoholic fatty liver disease (NAFLD) in SD rats. Addressing the terra incognita in DEHP-induced metabolic dysfunction, this study used HepG2 cells to investigate the potential mechanisms involved in DEHP-induced toxicity in vitro. The cells were established lipid overload model with oleic acid and BSA, then exposed to different concentrations (5, 10, 25, 50, 100 μmol/l DEHP) of DEHP for further analysis. The Oil Red O staining results showed that DEHP could promote lipid accumulation in cells. The level of superoxide dismutase (SOD) and malondialdehyde (MDA) changed suggested the balance of oxidative stress was disrupted. Additionally, western blot analysis showed that DEHP could promote the expression of peroxisome proliferator-activated receptor α (PPARα) and sterol regulatory element-binding protein 1c (SREBP-1c). By quantifying the expressions of the two proteins, it is of interest to determine that DEHP could promote lipid accumulation in hepatocytes via activating the SREBP-1c and PPARα-signaling pathway. |
Databáze: | OpenAIRE |
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