Plant-adapted Escherichia coli show increased lettuce colonizing ability, resistance to oxidative stress and chemotactic response
| Autor: | Juan Cesar Federico Ortiz-Marquez, Lina Lett, Maria de los Angeles Dublan, Leonardo Curatti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Physiology
LETTUCE lcsh:Medicine Plant Science COLONIZATON Biology medicine.disease_cause Plant Roots Microbiology Ciencias Biológicas purl.org/becyt/ford/1 [https] Escherichia coli medicine ADAPTATION purl.org/becyt/ford/1.6 [https] lcsh:Science Evolutionary Biology Multidisciplinary Ecology Plant roots Chemotaxis lcsh:R Biology and Life Sciences Agriculture Cell Biology Lettuce Bioquímica y Biología Molecular Plant Leaves Oxidative Stress Host-Pathogen Interactions lcsh:Q ESCHERICHIA COLI CIENCIAS NATURALES Y EXACTAS Research Article |
| Zdroj: | PLoS ONE, Vol 9, Iss 10, p e110416 (2014) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background: Escherichia coli is a widespread gut commensal and often a versatile pathogen of public health concern. E. coli are also frequently found in different environments and/or alternative secondary hosts, such as plant tissues. The lifestyle of E. coli in plants is poorly understood and has potential implications for food safety. Methods/Principal Findings: This work shows that a human commensal strain of E. coli K12 readily colonizes lettuce seedlings and produces large microcolony-like cell aggregates in leaves, especially in young leaves, in proximity to the vascular tissue. Our observations strongly suggest that those cell aggregates arise from multiplication of single bacterial cells that reach those spots. We showed that E. coli isolated from colonized leaves progressively colonize lettuce seedlings to higher titers, suggesting a fast adaptation process. E. coli cells isolated from leaves presented a dramatic rise in tolerance to oxidative stress and became more chemotactic responsive towards lettuce leaf extracts. Mutant strains impaired in their chemotactic response were less efficient lettuce colonizers than the chemotactic isogenic strain. However, acclimation to oxidative stress and/or minimal medium alone failed to prime E. coli cells for enhanced lettuce colonization efficiency. Conclusion/Significance: These findings help to understand the physiological adaptation during the alternative lifestyle of E. coli in/on plant tissues. Fil: Dublan, María de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina Fil: Ortiz Marquez, Juan César Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina Fil: Lett, Lina Analía Carola. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina Fil: Curatti, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina |
| Databáze: | OpenAIRE |
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