Enhanced production of anthraquinones by gamma-irradiated cell cultures of Rubia cordifolia in a bioreactor
| Autor: | Mariadoss A, Devanand P. Fulzele, Salma Younes, Ramesh K. Satdive, Siva Ramamoorthy, Hatem Zayed, George Priya Doss C, C. Rajasekaran |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Chromatography biology 010405 organic chemistry Rubia cordifolia Bioreactor Helical ribbon impeller Anthraquinones Gamma irradiation Alizarin biology.organism_classification Rushton disc turbine 01 natural sciences Anthraquinone 0104 chemical sciences chemistry.chemical_compound Murashige and Skoog medium chemistry Callus Subculture (biology) Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Industrial Crops and Products. 145:111987 |
| ISSN: | 0926-6690 |
| DOI: | 10.1016/j.indcrop.2019.111987 |
| Popis: | The aim of this study was to obtain high-yielding cell cultures of Rubia cordifolia by applying gamma irradiation and subsequently scaling up for anthraquinone production in a bioreactor. Calli cultured on MS medium was irradiated at variable doses between 2 Gy and 30 Gy. The callus cultures that were irradiated at 8 Gy accumulated a maximum alizarin level of 26.86 mg g-1 DW and a purpurin level of 44.85 mg g-1 DW during the M1T4 (fourth sub-cultures after gamma irradiation treatment) subculture, which was 6-fold and 11-fold higher than those of the nonirradiated callus cultures, respectively. Suspension cultures that originated from high-yielding callus cultures irradiated at 8 Gy were scaled up in an 8-L stirred bioreactor equipped with different impellers. The callus cultures in a bioreactor using a helical ribbon for homogenous mixing synthesized 63.58 % more anthraquinones than those in the bioreactor equipped with a Rushton disc turbine. This is the first report on the production of alizarin and purpurin in a bioreactor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect