Epigenetic Responses to Acute Resistance Exercise in Trained vs. Sedentary Men
| Autor: | Ryan T. McManus, Jared W. Coburn, Kyle J. Burghardt, Jose A. Arevalo, Andrew J. Galpin, Pablo B. Costa, James R. Bagley, Bradley H. Howlett, Moh H. Malek |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male medicine.medical_specialty Weight Lifting Skeletal muscle adaptation Physical Therapy Sports Therapy and Rehabilitation 030204 cardiovascular system & hematology Muscle hypertrophy Epigenesis Genetic Quadriceps Muscle 03 medical and health sciences Young Adult 0302 clinical medicine Internal medicine medicine Humans Orthopedics and Sports Medicine Epigenetics Muscle Strength Exercise physiology Leg press Muscle Skeletal Exercise computer.programming_language business.industry sed Interleukin-6 Tumor Necrosis Factor-alpha TOR Serine-Threonine Kinases Resistance Training 030229 sport sciences General Medicine Methylation DNA Methylation Endocrinology Long Interspersed Nucleotide Elements DNA methylation Sedentary Behavior business computer Proto-Oncogene Proteins c-akt Sterol Regulatory Element Binding Protein 2 |
| Zdroj: | Journal of strength and conditioning research. 34(6) |
| ISSN: | 1533-4287 |
| Popis: | Bagley, JR, Burghardt, KJ, McManus, R, Howlett, B, Costa, PB, Coburn, JW, Arevalo, JA, Malek, MH, and Galpin, AJ. Epigenetic responses to acute resistance exercise in trained vs. sedentary men. J Strength Cond Res 34(6): 1574-1580, 2020-Acute resistance exercise (RE) alters DNA methylation, an epigenetic process that influences gene expression and regulates skeletal muscle adaptation. This aspect of cellular remodeling is poorly understood, especially in resistance-trained (RT) individuals. The study purpose was to examine DNA methylation in response to acute RE in RT and sedentary (SED) young men, specifically targeting genes responsible for metabolic, inflammatory, and hypertrophic muscle adaptations. Vastus lateralis biopsies were performed before (baseline), 30 minutes after, and 4 hours after an acute RE bout (3 × 10 repetitions at 70% 1 repetition maximum [1RM] leg press and leg extension) in 11 RT (mean ± SEM: age = 26.1 ± 1.0 years; body mass = 84.3 ± 0.2 kg; leg press 1RM = 412.6 ± 25.9 kg) and 8 SED (age = 22.9 ± 1.1 years; body mass = 75.6 ± 0.3 kg; leg press 1RM = 164.8 ± 22.5 kg) men. DNA methylation was analyzed through methylation sensitive high-resolution melting using real-time polymerase chain reaction. Separate 2 (group) × 3 (time) repeated-measures analyses of variance and analyses of covariance were performed to examine changes in DNA methylation for each target gene. Results showed that acute RE (a) hypomethylated LINE-1 (measure of global methylation) in RT but not SED, (b) hypermethylated metabolic genes (GPAM and SREBF2) in RT, while lowering SREBF2 methylation in SED, and (c) did not affect methylation of genes associated with inflammation (IL-6 and TNF-α) or hypertrophy (mTOR and AKT1). However, basal IL-6 and TNF-α were lower in SED compared with RT. These findings indicate the same RE stimulus can illicit different epigenetic responses in RT vs. SED men and provides a molecular mechanism underpinning the need for differential training stimuli based on subject training backgrounds. |
| Databáze: | OpenAIRE |
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