RGD-Dendrimer-Poly(L-lactic) Acid Nanopatterned Substrates for the Early Chondrogenesis of Human Mesenchymal Stromal Cells Derived from Osteoarthritic and Healthy Donors

Autor: José Becerra, Francisco J. Blanco, Josep Samitier, José A. Andrades, Yolanda Vida, Joana Magalhães, Anna Lagunas, Ezequiel Perez-Inestrosa, Cristina Rodríguez-Pereira, Ignasi Casanellas
Rok vydání: 2020
Předmět:
RGD-density
Cell
Connexin
Artrosi
Cell condensation
02 engineering and technology
lcsh:Technology
Article
03 medical and health sciences
Medicina regenerativa
Dendrimer
Osteoarthritis
Gene expression
medicine
General Materials Science
chondrogenic differentiation
lcsh:Microscopy
Gap junctions
gap junctions
lcsh:QC120-168.85
030304 developmental biology
0303 health sciences
lcsh:QH201-278.5
lcsh:T
Chondrogenic differentiation
Chemistry
cell condensation
Mesenchymal stem cell
Gap junction
021001 nanoscience & nanotechnology
Chondrogenesis
Condrogènesi
Cell biology
osteoarthritis
Cell Pellet
medicine.anatomical_structure
lcsh:TA1-2040
Regenerative medicine
lcsh:Descriptive and experimental mechanics
lcsh:Electrical engineering. Electronics. Nuclear engineering
lcsh:Engineering (General). Civil engineering (General)
0210 nano-technology
lcsh:TK1-9971
Zdroj: Materials
Volume 13
Issue 10
RUC: Repositorio da Universidade da Coruña
Universidade da Coruña (UDC)
Dipòsit Digital de la UB
Universidad de Barcelona
RUC. Repositorio da Universidade da Coruña
Universitat Oberta de Catalunya (UOC)
Materials, Vol 13, Iss 2247, p 2247 (2020)
ISSN: 1996-1944
DOI: 10.3390/ma13102247
Popis: Aiming to address a stable chondrogenesis derived from mesenchymal stromal cells (MSCs) to be applied in cartilage repair strategies at the onset of osteoarthritis (OA), we analyzed the effect of arginine&ndash
glycine&ndash
aspartate (RGD) density on cell condensation that occurs during the initial phase of chondrogenesis. For this, we seeded MSC-derived from OA and healthy (H) donors in RGD-dendrimer-poly(L-lactic) acid (PLLA) nanopatterned substrates (RGD concentrations of 4 ×
10&minus
9, 10&minus
8, 2.5 ×
8, and 10&minus
2 w/w), during three days and compared to a cell pellet conventional three-dimensional culture system. Molecular gene expression (collagens type-I and II&ndash
COL1A1 and COL2A1, tenascin-TNC, sex determining region Y-box9-SOX9, and gap junction protein alpha 1&ndash
GJA1) was determined as well as the cell aggregates and pellet size, collagen type-II and connexin 43 proteins synthesis. This study showed that RGD-tailored first generation dendrimer (RGD-Cys-D1) PLLA nanopatterned substrates supported the formation of pre-chondrogenic condensates from OA- and H-derived human bone marrow-MSCs with enhanced chondrogenesis regarding the cell pellet conventional system (presence of collagen type-II and connexin 43, both at the gene and protein level). A RGD-density dependent trend was observed for aggregates size, in concordance with previous studies. Moreover, the nanopatterns&rsquo
had a higher effect on OA-derived MSC morphology, leading to the formation of bigger and more compact aggregates with improved expression of early chondrogenic markers.
Databáze: OpenAIRE
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