Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers
| Autor: | Ichiro Hirao, Yee Sin Leo, Ken-ichiro Matsunaga, Shawn Vasoo, Tun-Linn Thein, William Sun, Vanessa W. Lim, Michiko Kimoto |
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| Přispěvatelé: | Lee Kong Chian School of Medicine (LKCMedicine), National Centre for Infectious Diseases, Department of Infectious Diseases, Tan Tock Seng Hospital |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Serotype
Science Aptamer Antibody Affinity Diseases Enzyme-Linked Immunosorbent Assay DNA Aptamers Biology Cross Reactions Viral Nonstructural Proteins Antibodies Viral Serogroup Medical care Sensitivity and Specificity Article Serology Dengue fever Dengue Antigen medicine Humans Medicine [Science] Serologic Tests Antigens Viral Multidisciplinary Plasma samples Zika Virus Infection Biological techniques Biological Techniques Zika Virus Aptamers Nucleotide Dengue Virus medicine.disease Virology Immunoglobulin G Medicine |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-18 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Serologic tests to detect specific IgGs to antigens related to viral infections are urgently needed for diagnostics and therapeutics. We present a diagnostic method for serotype-specific IgG identification of dengue infection by a competitive enzyme-linked immunosorbent assay (ELISA), using high-affinity unnatural-base-containing DNA (UB-DNA) aptamers that recognize the four categorized serotypes. Using UB-DNA aptamers specific to each serotype of dengue NS1 proteins (DEN-NS1), we developed our aptamer-antibody sandwich ELISA for dengue diagnostics. Furthermore, IgGs highly specific to DEN-NS1 inhibited the serotype-specific NS1 detection, inspiring us to develop the competitive ELISA format for dengue serotype-specific IgG detection. Blood samples from Singaporean patients with primary or secondary dengue infections confirmed the highly specific IgG detection of this format, and the IgG production initially reflected the serotype of the past infection, rather than the recent infection. Using this dengue competitive ELISA format, cross-reactivity tests of 21 plasma samples from Singaporean Zika virus-infected patients revealed two distinct patterns: 8 lacked cross-reactivity, and 13 were positive with unique dengue serotype specificities, indicating previous dengue infection. This antigen-detection ELISA and antibody-detection competitive ELISA combination using the UB-DNA aptamers identifies both past and current viral infections and will facilitate specific medical care and vaccine development for infectious diseases. Agency for Science, Technology and Research (A*STAR) National Research Foundation (NRF) Published version This work was supported by the Institute of Bioengineering and Bioengineering (Biomedical Research Council, Agency for Science, Technology and Research, Singapore) and the National Research Foundation of Singapore (NRF-CRP17-2017-07). |
| Databáze: | OpenAIRE |
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